1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-16
  6. Animal-Free FGF-16 蛋白, Human (His)

Animal-Free FGF-16 蛋白, Human (His)

目录号: HY-P700059AF
COA 产品使用指南

FGF-16 蛋白协调胚胎发育,调节细胞增殖和分化。通过与 FGFR1FGFR2 的复杂相互作用,它在心肌细胞增殖和心脏发育中的重要作用变得显而易见。FGF-16 形成重要的分子连接,有助于对胚胎发育和细胞过程至关重要的信号通路。Animal-Free FGF-16 蛋白, Human (His) 是重组的 无动物源 FGF-16 蛋白,由 E. coli 表达,带有 C-His 标签。Animal-Free FGF-16 蛋白, Human (His) 全长 207 个氨基酸,分子量约为 ~24.57 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥900 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

FGF-16 Protein orchestrates embryonic development, regulating cell proliferation and differentiation. Its vital role in cardiomyocyte proliferation and heart development becomes evident through intricate interactions with FGFR1 and FGFR2. Forming essential molecular connections, FGF-16 contributes to signaling pathways crucial for embryonic development and cellular processes. Animal-Free FGF-16 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-16 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free FGF-16 Protein, Human (His) is 207 a.a., with molecular weight of ~24.57 kDa.

研究背景

FGF-16 蛋白在协调胚胎发育、对细胞增殖和分化等关键过程发挥调节影响方面发挥着关键作用。在心肌细胞增殖和心脏发育的复杂领域,FGF-16 发挥着至关重要的作用,其不可或缺性变得显而易见。该蛋白质与 FGFR1FGFR2 相互作用,形成重要的分子连接,有助于协调对胚胎发育和细胞过程至关重要的信号通路。

生物活性

Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <31 ng/mL. The specific activity of recombinant human FGF-16 is >3 x 104 IU/mg.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

O43320 (A1-R207)

基因 ID
蛋白结构
N-term
FGF-16 (A1-R207)
Accession # O43320
His
C-term
同用名
FGF-16; FGF-16; Fibroblast Growth Factor-16
氨基酸序列

MAEVGGVFASLDWDLHGFSSSLGNVPLADSPGFLNERLGQIEGKLQRGSPTDFAHLKGILRRRQLYCRTGFHLEIFPNGTVHGTRHDHSRFGILEFISLAVGLISIRGVDSGLYLGMNERGELYGSKKLTRECVFREQFEENWYNTYASTLYKHSDSERQYYVALNKDGSPREGYRTKRHQKFTHFLPRPVDPSKLPSMSRDLFHYR

分子量

Approximately 24.57 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 7.4.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free FGF-16 Protein, Human (His)
目录号:
HY-P700059AF
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