2. 重组蛋白
  3. CD Antigens
  4. Erythrocyte CD Proteins Endothelial cell CD Proteins
  5. CD297/ART4
  6. ART4/CD297 蛋白, Rat (HEK293, His)

ART4/CD297 蛋白, Rat (HEK293, His)

目录号: HY-P77301
产品使用指南

ART4/CD297 是一种预测的 NAD+ ADP-核糖基转移酶，在肾上腺和胸腺等组织中表现出偏向表达。作为人类 ART4 的直系同源物，它参与肽基精氨酸 ADP 核糖基化。ART4/CD297 蛋白, Rat (HEK293, His) 是重组的 ART4/CD297 蛋白，由 HEK293 表达，带有 C-His 标签。ART4/CD297 蛋白, Rat (HEK293, His) 全长 269 个氨基酸，分子量为 36-41 kDa。

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描述

ART4/CD297 is a predicted NAD+ ADP-ribosyltransferase that exhibits biased expression in tissues such as the adrenal gland and thymus. As an ortholog of human ART4, it is involved in peptidyl arginine ADP ribosylation. ART4/CD297 Protein, Rat (HEK293, His) is the recombinant rat-derived ART4/CD297 protein, expressed by HEK293 , with C-His labeled tag. The total length of ART4/CD297 Protein, Rat (HEK293, His) is 269 a.a., with molecular weight of 36-41 kDa.
研究背景

ART4/CD297 是一种预测的 NAD+ ADP-核糖基转移酶，与肽基-精氨酸 ADP-核糖基化有关。作为人类 ART4（ADP-核糖基转移酶 4（无活性）（Dombrock 血型））的直系同源物，该蛋白在肾上腺、胸腺和其他六种组织等组织中表现出偏向表达。NAD+ ADP-核糖基转移酶活性的预测强调了其在翻译后修饰过程中的潜在作用，特别是在肽基-精氨酸 ADP-核糖基化的背景下，有助于该酶的功能多样性。
种属

Rat
表达系统

HEK293
标签

C-His
蛋白编号

NP_001166980 (M1-K269)
基因 ID

312806  [NCBI]
蛋白结构
N-term
ART4 (M1-K269)
Accession # NP_001166980
His
C-term
同用名
Ecto-ADP-ribosyltransferase 4; ARTC4; Mono(ADP-ribosyl)transferase 4; DO; DOK1
分子量

36-41 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ART4/CD297 Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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