1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. B3GNT2 蛋白, Human (HEK293, Fc)

B3GNT2 蛋白, Human (HEK293, Fc)

目录号: HY-P76160
COA 产品使用指南

B3GNT2 蛋白是含锌乙醇脱氢酶家族的成员,有助于将醇氧化为其相应的羰基化合物。这种酶广泛分布于生物体中,其活性位点具有锌离子,通过脱氢催化底物转化。除了酒精代谢之外,B3GNT2 在多种生理过程中也至关重要,有助于酒精解毒和维持细胞氧化还原平衡。该家族的存在强调了其在不同生物系统中的进化意义和生理重要性。B3GNT2 蛋白, Human (HEK293, Fc) 是重组的 B3GNT2 蛋白,由 HEK293 表达,带有 N-hFc 标签。B3GNT2 蛋白, Human (HEK293, Fc) 全长 369 个氨基酸,分子量约为 ~95-130 kDa。

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10 μg ¥1360 In-stock
50 μg ¥3800 In-stock
100 μg ¥6460 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

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描述

B3GNT2 Protein, a member of the zinc-containing alcohol dehydrogenase family, is instrumental in oxidizing alcohols to their corresponding carbonyl compounds. Widely distributed across organisms, this enzyme, with a zinc ion in its active site, catalyzes substrate conversion through dehydrogenation. Beyond alcohol metabolism, B3GNT2 is crucial in diverse physiological processes, aiding alcohol detoxification and maintaining cellular redox balance. The family's presence underscores its evolutionary significance and physiological importance across different biological systems. B3GNT2 Protein, Human (HEK293, Fc) is the recombinant human-derived B3GNT2 protein, expressed by HEK293, with N-hFc labeled tag. The total length of B3GNT2 Protein, Human (HEK293, Fc) is 369 a.a., with molecular weight of ~95-130 kDa.

研究背景

醇脱氢酶是含锌醇脱氢酶家族的成员,在醇氧化为其相应的羰基化合物的过程中起着至关重要的作用。这种酶广泛分布于各种生物体中,促进乙醇和其他脂肪醇的代谢。锌离子是活性位点的结构成分,有助于通过脱氢反应催化底物的转化。除了在酒精代谢中发挥关键作用外,乙醇脱氢酶还参与多种生理过程,有助于酒精解毒和维持细胞氧化还原平衡。该酶家族的存在强调了其在不同生物系统中的进化意义和生理重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

N-hFc

蛋白编号

Q9NY97 (K29-C397)

基因 ID
蛋白结构
N-term
hFc
B3GNT2 (K29-C397)
Accession # Q9NY97
C-term
中文名
重组人B3GNT2 蛋白
同用名
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2; B3GALT7; B3GNT1
氨基酸序列

KSSSQEKNGKGEVIIPKEKFWKISTPPEAYWNREQEKLNRQYNPILSMLTNQTGEAGRLSNISHLNYCEPDLRVTSVVTGFNNLPDRFKDFLLYLRCRNYSLLIDQPDKCAKKPFLLLAIKSLTPHFARRQAIRESWGQESNAGNQTVVRVFLLGQTPPEDNHPDLSDMLKFESEKHQDILMWNYRDTFFNLSLKEVLFLRWVSTSCPDTEFVFKGDDDVFVNTHHILNYLNSLSKTKAKDLFIGDVIHNAGPHRDKKLKYYIPEVVYSGLYPPYAGGGGFLYSGHLALRLYHITDQVHLYPIDDVYTGMCLQKLGLVPEKHKGFRTFDIEEKNKNNICSYVDLMLVHSRKPQEMIDIWSQLQSAHLKC

分子量

Approximately 95-130 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

B3GNT2 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
B3GNT2 Protein, Human (HEK293, Fc)
目录号:
HY-P76160
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