1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 2 (CA-II)
  5. Carbonic Anhydrase 2 蛋白, Mouse (His)

Carbonic Anhydrase 2 蛋白, Mouse (His)

目录号: HY-P72861
COA 产品使用指南

Carbonic Anhydrase 2 蛋白是碳酸酐酶同工酶家族的成员,催化二氧化碳的可逆水合。CA2 突变与骨硬化症和肾小管酸中毒有关。该基因在多种组织中表现出偏向表达,特别是在胃和结肠中。CA2 在离子传输和 pH 平衡的调节中起着至关重要的作用,并参与许多生物过程。Carbonic Anhydrase 2 蛋白, Mouse (His) 是重组的 碳酸酐酶 2 蛋白,由 HEK293 表达,带有 C-His 标签。Carbonic Anhydrase 2 蛋白, Mouse (His) 全长 259 个氨基酸,分子量为 30-35 kDa。

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描述

Carbonic Anhydrase 2 Protein is a member of the carbonic anhydrase isozyme family, responsible for catalyzing the reversible hydration of carbon dioxide. Mutations in CA2 are linked to osteopetrosis and renal tubular acidosis. The gene exhibits biased expression in various tissues, notably in the stomach and colon. CA2 plays vital role in the regulation of ion transport and pH balance and is involved in many biological processes. Carbonic Anhydrase 2 Protein, Mouse (His) is the recombinant mouse-derived Carbonic Anhydrase 2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Carbonic Anhydrase 2 Protein, Mouse (His) is 259 a.a., with molecular weight of 30-35 kDa.

研究背景

碳酸酐酶 2 (CA2) 是碳酸酐酶同工酶家族的成员,负责催化二氧化碳的可逆水合。这种酶的失调与骨硬化症和肾小管酸中毒等疾病有关。已鉴定出编码不同亚型的两种转录变体。除了在二氧化碳代谢中的基本作用外,该蛋白还在各种组织中表现出偏向表达,其中在胃和结肠以及其他八个组织中表达水平显著。它还可以将氰胺水合成尿素。CA2 对于骨吸收和破骨细胞分化至关重要。CA2 在离子传输和 pH 平衡的调节中起着至关重要的作用,并参与许多生物过程。此外,CA2下调促进HCC转移和侵袭。它可作为 HCC 转移和 EMT 的抑制剂,并与 HCC 患者良好的总生存 (OS) 相关[1][2][3][4]

生物活性

Measured by its esterase activity. The specific activity is > 400 pmol/min/µg.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

AAH55291 (S2-K260)

基因 ID
蛋白结构
N-term
CA2 (S2-K260)
Accession # AAH55291
His
C-term
同用名
Carbonic anhydrase 2; Carbonic anhydrase C; CAC; CA-II; CA2
氨基酸序列

SHHWGYSKHNGPENWHKDFPIANGDRQSPVDIDTATAQHDPALQPLLISYDKAASKSIVNNGHSFNVEFDDSQDNAVLKGGPLSDSYRLIQFHFHWGSSDGQGSEHTVNKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKIGPASQGLQKVLEALHSIKTKGKRAAFANFDPCSLLPGNLDYWTYPGSLTTPPLLECVTWIVLREPITVSSEQMSHFRTLNFNEEGDAEEAMVDNWRPAQPLKNRKIKASFK

分子量

30-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as 0.22 μm filtered solution in PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
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目录号:
HY-P72861
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