CD19 蛋白, Human (CHO, Fc)

CD19 Protein, Human (CHO, Fc) is a polypeptide chain with the C-terminal human IgG1 Fc fragment produced in CHO cells. CD19 is a biomarker for normal and neoplastic B cells.

CD19被归类为I型跨膜蛋白，具有单个跨膜结构域、胞质C端和胞外N端。CD19 是正常和肿瘤性 B 细胞以及滤泡树突状细胞的生物标志物。CD19 通过调节 B 细胞受体依赖性和独立信号传导，关键参与建立内在 B 细胞信号传导阈值。CD19 与补体受体 CD21、四跨膜蛋白 CD81 (TAPA-1) 以及 CD225 一起，作为成熟 B 细胞表面多分子复合物的主要信号传导成分。通过对 CD19 转基因和敲除小鼠模型的研究，我们发现 CD19 在维持体液、抗原诱导反应和耐受诱导之间的平衡中发挥着关键作用^[1]。

Human

CHO

C-hFc

P15391 (P20-K291)

930  [NCBI]

PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK

Approximately 62 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang K, et al. CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol. 2012 Nov 29;1(1):36.  [Content Brief]