1. 重组蛋白
  2. CAR-T related Proteins CD Antigens Biotinylated Proteins
  3. T Cell CD Proteins B Cell CD Proteins Stem Cell CD Proteins
  4. CD38
  5. CD38 蛋白, Human (Biotinylated, HEK293, His-Avi)

CD38 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P72356
COA 产品使用指南

CD38 蛋白在细胞信号传导中发挥着关键作用,能够熟练地合成环状 ADP-核糖 (cADPR),作为葡萄糖诱导的胰岛素分泌的关键第二信使。它还促进钙动员剂烟酸-腺嘌呤二核苷酸磷酸 (NAADP+) 的合成。CD38 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 CD38 蛋白,由 HEK293 表达,带有 N-Avi, N-6*His 标签。CD38 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 258 个氨基酸,分子量为 40-50 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥2930 In-stock
100 μg ¥8200 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CD38 protein plays a key role in cell signaling, proficiently synthesizing cyclic ADP-ribose (cADPR) as a critical second messenger for glucose-induced insulin secretion. It also promotes the synthesis of the calcium mobilizer niacin-adenine dinucleotide phosphate (NAADP+). CD38 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD38 protein, expressed by HEK293 , with N-Avi, N-6*His labeled tag. The total length of CD38 Protein, Human (Biotinylated, HEK293, His-Avi) is 258 a.a., with molecular weight of 40-50 kDa.

研究背景

CD38 蛋白在细胞信号传导中发挥着关键作用,是环状 ADP-核糖 (cADPR) 的熟练合成者,而 cADPR 是公认的对葡萄糖诱导的胰岛素分泌至关重要的第二信使。此外,它还促进钙动员剂烟酸-腺嘌呤二核苷酸磷酸、NAADP(+) 的合成,NAADP(+) 源自 2'-磷酸-cADPR 和烟酸,以及 NADP(+) 和烟酸。在 pH 5.0 和 pH 7.4 下工作时,CD38 蛋白优先将 2'-磷酸-cADPR 转化为 NAADP(+),同时选择性地将 NADP(+) 裂解为 cADPR 和 ADPRP,而不是生成 NADDP(+)。值得注意的是,它还展示了 cADPR 水解酶活性,突出了其在这些关键信号分子动态调节中的多方面作用。这些活性的整合强调了 CD38 蛋白在调节细胞反应中的重要性,特别是在胰岛素分泌和钙动员的背景下。

生物活性

1.Immobilized Anti-Human CD38 mAb at 2 μg/mL (100 μL/well) can bind Human CD38-His-Avi. The ED50 of Human CD38-His-Avi is 13-24.54 ng/mL.
2.Loaded Biotinylated Human CD38-His on HIS1K Biosensor, can bind Anti-Human CD38 mAb-Fc with an affinity constant of 0.006 nM as determined in BLI assay.

种属

Human

表达系统

HEK293

标签

N-Avi;N-6*His

蛋白编号

P28907 (V43-I300)

基因 ID

952  [NCBI]

蛋白结构
N-term
6*His-Avi
CD38 (V43-I300)
Accession # P28907
C-term
同用名
ADP-ribosyl cyclase1; 2'-phospho-ADP-ribosyl cyclase; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1
氨基酸序列

VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI

分子量

40-50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
CD38 Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P72356
需求量: