1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. cGAS 蛋白, Human (His)

cGAS 蛋白, Human (His)

目录号: HY-P72337
COA 产品使用指南

cGAS 蛋白是一种核苷酸转移酶,在与双链 DNA (dsDNA) 结合后催化 ATP 和 GTP 形成 2',3'-cGAMP,从而对先天免疫发挥至关重要的介导作用。作为 DNA 传感器,cGAS 检测外源性和内源性 DNA,触发 STING1 激活和 I 型干扰素产生。cGAS 蛋白, Human (His) 是重组的 cGAS 蛋白,由 E. coli 表达,带有 C-6*His 标签。cGAS 蛋白, Human (His) 全长 522 个氨基酸,分子量约为 ~58.9 kDa。

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描述

The cGAS protein is a nucleotidyl transferase that crucially mediates innate immunity by catalyzing the formation of 2',3'-cGAMP from ATP and GTP upon binding to double-stranded DNA (dsDNA). As a DNA sensor, cGAS detects exogenous and endogenous DNA, triggering STING1 activation and type I interferon production. cGAS Protein, Human (His) is the recombinant human-derived cGAS protein, expressed by E. coli , with C-6*His labeled tag. The total length of cGAS Protein, Human (His) is 522 a.a., with molecular weight of ~58.9 kDa.

研究背景

cGAS 蛋白是一种核苷酸转移酶,通过催化 ATP 和 GTP 形成环 GMP-AMP (2',3'-cGAMP),在先天免疫中发挥关键作用。作为关键的 DNA 传感器,cGAS 直接结合双链 DNA (dsDNA),诱导 2',3'-cGAMP 的合成,这是激活 STING1 的第二信使,从而产生 I 型干扰素并触发免疫反应。cGAS 可识别弯曲的长 dsDNA,充当外来 DNA 传感器,检测来自病毒、逆转录病毒、细菌和中性粒细胞胞外陷阱 (NET) 的 DNA 的存在。此外,cGAS 对细胞应激过程中释放的内源 DNA 做出反应,导致无菌炎症。它通过与胞质染色质片段结合来调节细胞衰老,并参与对基因组不稳定和双链 DNA 断裂的炎症反应。cGAS 还参与感知翻译应激,其激活会抑制 DNA 损伤时的同源重组修复。人类 cGAS 表现出物种特异性的 DNA 识别机制,可以对病原体做出更精细的反应。

生物活性

Enzyme activity has been tested in vitro.

种属

Human

表达系统

E. coli

标签

C-6*His

蛋白编号

Q8N884-1 (M1-F522)

基因 ID
蛋白结构
N-term
cGAS (M1-F522)
Accession # Q8N884
6*His
C-term
同用名
Cyclic GMP-AMP synthase; cGAMP synthase; h-cGAS; 2'3'-cGAMP synthase; Mab-21 domain-containing protein 1; MB21D1; C6orf150
氨基酸序列

MQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGSCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF

分子量

Approximately 58.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris HCl, 100 mM NaCl, 1 mM DTT, 5% Glycerol, pH 7.5 or PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

cGAS Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
cGAS Protein, Human (His)
目录号:
HY-P72337
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