1. 重组蛋白
  2. Receptor Proteins
  3. Pattern Recognition Receptors
  4. C-type Lectin Receptors
  5. C-Type Lectin Domain Family 10 Member A/CD301
  6. CLEC10A/CD301 蛋白, Mouse (HEK293, Fc)

CLEC10A/CD301 蛋白, Mouse (HEK293, Fc)

目录号: HY-P76265
COA 产品使用指南

CLEC10A/CD301 预测了碳水化合物结合活性,并在炎症反应和伤口愈合的结缔组织替代中发挥上游作用。这种蛋白质预计位于膜上,并且是膜结构的一个组成部分,在质膜外侧活跃。CLEC10A/CD301 蛋白, Mouse (HEK293, Fc) 是重组的 CLEC10A/CD301 蛋白,由 HEK293 表达,带有 N-hFc 标签。CLEC10A/CD301 蛋白, Mouse (HEK293, Fc) 全长 248 个氨基酸,分子量约为 ~58 KDa。

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5 μg ¥630 In-stock
10 μg ¥1070 In-stock
50 μg ¥3000 In-stock
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500 μg ¥14300 In-stock
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  • 生物活性

  • 技术参数

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描述

CLEC10A/CD301 has predicted carbohydrate-binding activity and plays an upstream role in connective tissue replacement in inflammatory responses and wound healing. This protein is expected to be located on the membrane and be an integral part of the membrane structure, being active on the outside of the plasma membrane. CLEC10A/CD301 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CLEC10A/CD301 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CLEC10A/CD301 Protein, Mouse (HEK293, Fc) is 248 a.a., with molecular weight of ~58 KDa.

研究背景

CLEC10A/CD301 是一种具有预测碳水化合物结合活性的蛋白质,在参与炎症反应和伤口愈合的结缔组织替代上游发挥作用。预计该蛋白质位于膜中并作为膜结构的组成部分,预计在质膜的外侧具有活性。CLEC10A在多种结构中检测到表达,包括软骨、真皮、胚胎间质、第四脑室脉络丛的后脑部分和脊髓边缘层。CLEC10A 在乳腺 (RPKM 20.4) 和膀胱 (RPKM 15.0) 等组织中的偏向表达表明它可能参与不同组织的不同生理过程。

种属

Mouse

表达系统

HEK293

标签

N-hFc

蛋白编号

NP_001191181.1 (Q58-S305)

基因 ID

17312  [NCBI]

蛋白结构
N-term
hFc
CLEC10A (Q58-S305)
Accession # NP_001191181.1
C-term
同用名
C-type lectin domain family 10 member A; CD301; CLEC10A; CLECSF13; CLECSF14; HML
氨基酸序列

QNSQLRRDLGTLRATLDNTTSKIKAEFQSLDSRADSFEKGISSLKVDVEDHRQELQAGRDLSQKVTSLESTVEKREQALKTDLSDLTDHVQQLRKDLKALTCQLANLKNNGSEVACCPLHWTEHEGSCYWFSESEKSWPEADKYCRLENSHLVVVNSLEEQNFLQNRLANVVSWIGLTDQNGPWRWVDGTDFEKGFKNWAPLQPDNWFGHGLGGGEDCAHITTGGPWNDDVCQRTFRWICEMKLAKES

分子量

Approximately 58 kDa.

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CLEC10A/CD301 Protein, Mouse (HEK293, Fc)
目录号:
HY-P76265
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