Cofilin-1 蛋白, Human (His)

Cofilin-1 protein has pH-sensitive F-actin depolymerizing activity and can bind to F-actin. It cooperates with the subcortical maternal complex (SCMC) to guide the fertilized egg through initial embryonic cell division by regulating actin dynamics. Cofilin-1 Protein, Human (His) is the recombinant human-derived Cofilin-1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Cofilin-1 Protein, Human (His) is 166 a.a., with molecular weight of ~21 kDa.

Cofilin-1 蛋白通过与 F-肌动蛋白结合而表现出 pH 敏感的 F-肌动蛋白解聚活性。它与皮层下母体复合体 (SCMC) 合作，通过调节肌动蛋白动力学，在使受精卵超越初始胚胎细胞分裂方面发挥着至关重要的作用。此外，Cofilin-1 对于有丝分裂纺锤体的集中和受精卵的对称分裂至关重要。在上皮细胞中，它有助于细胞形态和细胞骨架组织的调节。此外，Cofilin-1 是非典型趋化因子受体 ACKR2 上调所必需的，促进其从内体区室有效易位至细胞膜，从而增强趋化因子的摄取和降解。它的参与延伸到神经管形态发生和神经嵴细胞迁移。在功能上，Cofilin-1可以以1:1的比例结合G-和F-肌动蛋白，构成核内和细胞质肌动蛋白杆的主要成分。与皮质下母体复合体的相互作用涉及 TLE6 同工型 1 和 NLRP5，以及与 C9orf72 的相互作用。

Human

E. coli

N-6*His

P23528 (M1-L166)

1072  [NCBI]

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Approximately 21 kDa

• 
  Greater than 90 % as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.