1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory checkpoint molecules T Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins
  4. CTLA-4 CD152/CTLA-4
  5. CTLA-4 蛋白, Mouse (125a.a, HEK293, His)

CTLA-4 蛋白, Mouse (125a.a, HEK293, His)

目录号: HY-P71991
COA 产品使用指南

CTLA-4 蛋白, Mouse (125a.a, HEK 293, His) 是带有 His 标签的重组 CTLA-4 蛋白。CTLA-4 蛋白是一种单通道 I 型膜蛋白,属于 CD28 受体家族。CTLA-4 是一种阴性免疫调节剂,在 Treg 细胞上组成表达。

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规格 价格 是否有货 数量
2 μg ¥530 In-stock
10 μg ¥1350 In-stock
50 μg ¥3680 In-stock
100 μg   询价  
250 μg   询价  

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描述

CTLA-4 Protein, Mouse (125a.a, HEK 293, His) is a recombinant CTLA-4 protein with a His-flag. CTLA-4 Protein is a single-pass type I membrane protein and belongs to the CD28 receptor family. CTLA-4 is a negative immune regulator constitutively expressed on Treg cells[1][2].

研究背景

CTLA-4 and CD28 both belongs to a family of immunoglobulin-related receptors that are responsible for various aspects of T-cell immune regulation. CTLA-4 and CD28 are homologous receptors expressed by both CD4+ and CD8+ T cells, which mediate opposing functions in T cell activation[1].
CTLA-4 interacts with both ligands (CD80 and CD86) with high affinity[1].
CTLA-4 is a negative immune regulator constitutively expressed on Treg cells and upregulated on activated T cells. CTLA-4 inhibits T cell activation by various suppressive functions including competition with CD28, regulation of the inhibitory function of Treg cells, such as transendocytosis, and the control of adhesion and motility[1].

生物活性

The ED50 as determined by its ability to bind Mouse B7-1 in functional ELISA is less than 20 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P09793 (A37-D161)

基因 ID

12477  [NCBI]

同用名
Ctla4; Cd152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152
氨基酸序列

AIQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIDPEPCPDSD

分子量

Approximately 14.6 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm solution of 1xPBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CTLA-4 Protein, Mouse (125a.a, HEK293, His)
目录号:
HY-P71991
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