CXCL16 蛋白, Human (HEK293, His)

CXCL16, also known as SR-PSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein), is one member of the ELR-negative CXC chemokine subfamily. CXCL16 is a multifunctional protein involved in various inflammatory diseases, atherosclerosis, and cancer. CXCL16 can be observed in many cell types^[1][2]. CXCL16 Protein, Human (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of 224 amino acids (M1-T224).

CXCL16 是一种膜结合趋化因子。CXCL16以可溶性或跨膜形式表达，可在多种细胞类型中观察到，包括炎症细胞（如巨噬细胞、中性粒细胞、树突状细胞和单核细胞）和非炎症细胞（如肺上皮细胞和肾细胞）。CXCL16在天然免疫屏障和自身免疫性疾病的发生发展中发挥着重要作用^[1][2]。
CXCL16 蛋白在小鼠、大鼠和狗 CXCL16 蛋白之间具有较低的同源性。
CXCL16 主要表达于抗原呈递细胞 (APC) 表面，由趋化因子结构域（约 89 个氨基酸）组成，是一种粘蛋白型茎（约 110 个氨基酸）、单次跨膜结构域（约 20 个氨基酸）和胞质尾（约 27 个氨基酸）。CXCL16是CXCR6受体的唯一配体。可溶性CXCL16诱导CXCR6+细胞（包括Th1细胞、NK细胞和活化的CD8+T细胞）的迁移、M2巨噬细胞浸润、APC和CD8+T细胞之间的相互作用、细胞免疫反应和炎症反应以及发育胸腺细胞。膜结合的CXCL16可以促进CXCR6+细胞的粘附。CXCL16 特异性结合氧化低密度脂蛋白 (OxLDL)，导致其内化和降解。CXCL16可能在动脉粥样硬化病变的形成中发挥重要作用。巨噬细胞和树突状细胞上的 CXCL16 介导大肠杆菌和金黄色葡萄球菌等细菌的粘附和吞噬作用，而细菌识别则由 CXCL16 的趋化因子结构域介导 ^[1][2]。
CXCL16不仅是一种趋化因子，也是一种多功能蛋白。CXCL16和CXCR6与多种炎症性疾病有关，例如肾小球肾炎、肺部疾病、动脉粥样硬化、冠状动脉疾病、类风湿性关节炎和许多与炎症相关的癌症。CXCL16 的趋化因子结构域对大肠杆菌发挥有效的抗微生物活性。大肠杆菌和S。金黄色葡萄球菌。CXCL16 通过吸引表达 CXCR6 的细胞（例如激活的 T 细胞和 NKT 细胞）充当先天免疫的介质。CXCL16 也是人类表皮角质形成细胞先天免疫反应的新型介质^[1][2][3]。

Recombinant human CXCL16 (1 ng/mL) induces cell migration in primary human monocytes or M2 macrophages^[3].

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6 and the ED₅₀ is 50-250 ng/mL.

Human

HEK293

C-His

Q9H2A7 (N30-T205)

58191  [NCBI]

MSGSQSEVAPSPQSPRSPEMGRDLRPGSRVLLLLLLLLLVYLTQPGNGNEGSVTGSCYCGKRISSDSPPSVQFMNRLRKHLRAYHRCLYYTRFQLLSWSVCGGNKDPWVQELMSCLDLKECGHAYSGIVAHQKHLLPTSPPISQASEGASSDIHTPAQMLLSTLQSTQRPTLPVGSLSSDKELTRPNETTIHTAGHSLAAGPEAGENQKQPEKNAGPTARTSAT

Approximately 20.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Tohyama M, et al. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes. Int Immunol. 2007 Sep;19(9):1095-102.  [Content Brief]

  [2]. Jianhui Sun, et al. A Functional Variant of CXCL16 Is Associated With Predisposition to Sepsis and MODS in Trauma Patients: Genetic Association Studies. Front Genet. 2021 Sep 3;12:720313.  [Content Brief]

  [3]. Allaoui R, et al. Cancer-associated fibroblast-secreted CXCL16 attracts monocytes to promote stroma activation in triple-negative breast cancers. Nat Commun. 2016 Oct 11;7:13050.  [Content Brief]