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  3. CYGB 蛋白, Rat (His-Myc)

CYGB 蛋白, Rat (His-Myc)

目录号: HY-P72162
COA 产品使用指南

CYGB 蛋白在细胞防御氧化应激中发挥着至关重要的作用,并且可以作为减轻损伤的保护剂。它涉及与氧储存或转移相关的细胞内过程。CYGB 蛋白, Rat (His-Myc) 是重组的 CYGB 蛋白,由 E. coli 表达,带有 N-10*His, C-Myc 标签。CYGB 蛋白, Rat (His-Myc) 全长 190 个氨基酸,分子量约为 ~31 kDa。

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描述

CYGB proteins play a crucial role in cellular defense against oxidative stress and may serve as protective agents that mitigate damage. It involves intracellular processes related to oxygen storage or transfer. CYGB Protein, Rat (His-Myc) is the recombinant rat-derived CYGB protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of CYGB Protein, Rat (His-Myc) is 190 a.a., with molecular weight of ~31 kDa.

研究背景

CYGB 蛋白似乎在细胞防御氧化应激中发挥着关键作用,可能充当减轻损伤的保护剂。此外,它可能在与氧储存或转移相关的细胞内过程中发挥作用。值得注意的是,CYGB 与肝纤维化的发展有关,表明它与病理状况有关。此外,该蛋白质还具有过氧化物酶活性,强调其参与氧化还原反应并有助于细胞稳态的能力。

种属

Rat

表达系统

E. coli

标签

N-10*His;C-Myc

蛋白编号

Q921A4 (M1-P190)

基因 ID

170520  [NCBI]

蛋白结构
N-term
10*His
CYGB (M1-P190)
Accession # Q921A4
C-term
同用名
Cygb; StapCytoglobin; Histoglobin; HGb; Stellate cell activation-associated protein
氨基酸序列

MEKVPGDMEIERRERNEELSEAERKAVQATWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPMYFKILSGVILDVIAEEFANDFPVETQKAWTKLRGLIYSHVTAAYKEVGWVQQVPNTTTLPATLPSSGP

分子量

Approximately 31 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CYGB Protein, Rat (His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CYGB Protein, Rat (His-Myc)
目录号:
HY-P72162
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