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  4. Cytochrome b5/CYB5A 蛋白, Human (His)

Cytochrome b5/CYB5A 蛋白, Human (His)

目录号: HY-P7858
产品使用指南

细胞色素 b5 (CYB5A) 是一种重要的膜结合血红素蛋白，充当多种膜结合加氧酶的电子载体。它位于细胞膜中，对电子传递过程至关重要，支持加氧酶的活性。CYB5A 作为电子载体的作用凸显了其在细胞氧化还原反应和涉及加氧酶的代谢途径中的重要性。Cytochrome b5/CYB5A 蛋白, Human (His) 是重组的 细胞色素 b5/CYB5A 蛋白，由 E. coli 表达，带有 N-6*His 标签。Cytochrome b5/CYB5A 蛋白, Human (His) 全长 134 个氨基酸，分子量约为 ~17.0 kDa。

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描述

Cytochrome b5 (CYB5A) is a vital membrane-bound hemoprotein acting as an electron carrier for diverse membrane-bound oxygenases. Positioned in cellular membranes, it crucially facilitates electron transfer processes, supporting the activity of oxygenase enzymes. CYB5A's role as an electron carrier highlights its significance in cellular redox reactions and metabolic pathways involving oxygenases. Cytochrome b5/CYB5A Protein, Human (His) is the recombinant human-derived Cytochrome b5/CYB5A protein, expressed by E. coli , with N-6*His labeled tag. The total length of Cytochrome b5/CYB5A Protein, Human (His) is 134 a.a., with molecular weight of ~17.0 kDa.
研究背景

细胞色素 b5 (CYB5A) 是一种膜结合血红素蛋白，可作为各种膜结合加氧酶的电子载体。这种蛋白质位于细胞膜内，在促进电子传递过程中发挥着至关重要的作用，有助于加氧酶的活性。它作为电子载体的功能强调了它在涉及加氧酶的细胞氧化还原反应和代谢途径中的重要性。
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

P00167 (M1-D134)
基因 ID
蛋白结构
N-term
6*His
CYB5A (M1-D134)
Accession # P00167
C-term
同用名
rHuCytochrome b5/CYB5A, His; Cytochrome b5; Microsomal Cytochrome b5 Type A; MCB5; CYB5A; CYB5
氨基酸序列

MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

分子量

Approximately 17.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Cytochrome b5/CYB5A Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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