Cytochrome c/CYCS 蛋白, Human (His)

Cytochrome c is an electron carrier protein that accepts electrons from cytochrome c1 and transfers them to the cytochrome oxidase complex in the mitochondrial electron transport chain. It is involved in apoptosis, where the release of cytochrome c into the cytosol leads to caspase-9 activation and subsequent acceleration of apoptosis. Cytochrome c/CYCS Protein, Human (His) is the recombinant human-derived Cytochrome c/CYCS protein, expressed by E. coli , with C-6*His labeled tag. The total length of Cytochrome c/CYCS Protein, Human (His) is 104 a.a., with molecular weight of ~16.0 kDa.

细胞色素 c/CYCS 蛋白在细胞过程中充当重要的电子载体，特别是在线粒体电子传输链中。在其氧化形式下，细胞色素 c 血红素基团善于接受来自细胞色素还原酶的细胞色素 c1 亚基的电子。随后，该电子被转移到细胞色素氧化酶复合物，作为线粒体电子传递链中的最终蛋白质载体。除了在电子传递中的作用外，细胞色素 c 在细胞凋亡中也发挥着至关重要的作用，其中通过抑制 Bcl-2 家族的抗凋亡成员或激活促凋亡成员而引起的线粒体膜通透性的改变，导致细胞色素 c 的释放。细胞色素c进入细胞质。细胞色素 c 与 Apaf-1 的结合会触发 caspase-9 的激活，从而启动级联反应，通过激活其他 caspase 来加速细胞凋亡。这种双重功能强调了细胞色素 c/CYCS 蛋白在能量代谢和程序性细胞死亡调节中的多功能和关键作用。

Human

E. coli

C-6*His

P99999 (G2-E105)

54205  [NCBI]

GDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

Approximately 16.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 10% Trehalose, 200 mM NaCl, 50% Glycerol, 0.05% Tween 80, pH7.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.