1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. DCK/Deoxycytidine kinase 蛋白, Human (His-T7)

DCK/Deoxycytidine kinase 蛋白, Human (His-T7)

目录号: HY-P70019
COA 产品使用指南

DCK/脱氧胞苷激酶蛋白可有效磷酸化脱氧核糖核苷,包括脱氧胞苷、脱氧鸟苷和脱氧腺苷,表现出广泛的底物特异性,无手性选择性。作为一种不可或缺的酶,DCK 在磷酸化核苷类似物方面发挥着至关重要的作用,这在抗病毒和化疗治疗中至关重要。DCK/Deoxycytidine kinase 蛋白, Human (His-T7) 是重组的 DCK/脱氧胞苷激酶蛋白,由 E. coli 表达,带有 N-6*His, N-T7 标签。DCK/Deoxycytidine kinase 蛋白, Human (His-T7) 全长 260 个氨基酸,分子量约为 ~32.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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描述

DCK/deoxycytidine kinase protein can effectively phosphorylate deoxyribonucleosides, including deoxycytidine, deoxyguanosine and deoxyadenosine, showing broad substrate specificity and no chiral selectivity. As an indispensable enzyme, DCK plays a crucial role in phosphorylating nucleoside analogues, which is critical in antiviral and chemotherapeutic treatments. DCK/Deoxycytidine kinase Protein, Human (His-T7) is the recombinant human-derived DCK/Deoxycytidine kinase protein, expressed by E. coli , with N-6*His, N-T7 labeled tag. The total length of DCK/Deoxycytidine kinase Protein, Human (His-T7) is 260 a.a., with molecular weight of ~32.0 kDa.

研究背景

DCK 被称为脱氧胞苷激酶,通过有效磷酸化脱氧核糖核苷(包括脱氧胞苷、脱氧鸟苷和脱氧腺苷)来展示其酶促能力。该激酶具有广泛的底物特异性,不会表现出基于底物手性的选择性。其重要性延伸至成为常用作抗病毒和化疗剂的各种核苷类似物磷酸化不可或缺的酶。DCK 催化核苷磷酸化的能力在细胞过程中起着至关重要的作用,并有助于基于核苷类似物的治疗的疗效。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His;N-T7

蛋白编号

P27707 (M1-L260)

基因 ID
蛋白结构
N-term
6*His-T7
DCK (M1-L260)
Accession # P27707
C-term
同用名
rHuDeoxycytidine kinase/DCK, His-T7; Deoxycytidine Kinase; dCK; DCK
氨基酸序列

MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

分子量

Approximately 32.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 1 mM TCEP, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

DCK/Deoxycytidine kinase Protein, Human (His-T7) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DCK/Deoxycytidine kinase Protein, Human (His-T7)
目录号:
HY-P70019
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