Doublecortin/DCX 蛋白, Human (His)

Doublecortin/DCX Protein, a microtubule-associated protein, is vital for early stages of neuronal dispersion and cortex lamination during cerebral cortex development. It may compete with the neuronal protein kinase DCLK1 in binding to a target protein, contributing to crucial signaling pathways involved in neuronal interaction and migration. DCX interacts with tubulin and USP9X. Doublecortin/DCX Protein, Human (His) is the recombinant human-derived Doublecortin/DCX protein, expressed by E. coli , with N-GST labeled tag. The total length of Doublecortin/DCX Protein, Human (His) is 106 a.a., with molecular weight of ~13 kDa.

Doublecortin/DCX 蛋白作为微管相关蛋白，在大脑皮层发育过程中神经元分散和皮层层压的早期阶段发挥着关键作用。其功能可能涉及与假定的神经元蛋白激酶 DCLK1 竞争与靶蛋白的结合，从而参与迁移前和迁移过程中神经元相互作用所必需的关键信号通路。这个过程可能是钙离子依赖性信号转导途径的一部分。Doublecortin/DCX 还可能与 PAFAH1B1/LIS-1 合作，促成重叠但不同的信号通路，共同促进有效的神经元迁移。在这种复杂的细胞编排中，Doublecortin/DCX 与微管蛋白和 USP9X 相互作用，强调其参与微管动力学的调节，并强调其在控制神经元发育的复杂分子途径中的重要性。

Human

E. coli

N-6*His

O43602-2 (A45-V150)

1641  [NCBI]

ALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNV

Approximately 13 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.