2. 重组蛋白
  3. Receptor Proteins
  4. G-Protein-Coupled Receptors (GPCRs)
  5. Endothelin Receptor
  6. Endothelin R Type B (EDNRB)
  7. EDNRB/Endothelin R Type B Protein-VLP, Human (HEK293, GFP)

EDNRB/Endothelin R Type B Protein-VLP, Human (HEK293, GFP)

目录号: HY-P704572
产品使用指南 技术支持

EDNRB/内皮素 R B 型蛋白是内皮素 1、2 和 3 的非特异性受体，通过 G 蛋白激活磷脂酰肌醇-钙第二信使系统。这一机制强调了其在介导内皮素神经肽引发的细胞反应中的关键作用，强调了 EDNRB 在转导各种生理过程信号中的多功能性。EDNRB/Endothelin R Type B Protein-VLP, Human (HEK293, GFP) 是重组的 EDNRB/Endothelin R Type B 蛋白，由 HEK293 表达，带有 GFP 标签。VLPs在HEK293细胞培养中形成，将膜蛋白集中在细胞表面，产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强，需优化免疫策略，包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰。SPR/BLI分析则对芯片和实验方案有较高要求，成功率较低。如果需要VLP对照，推荐HY-P705435。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μg ￥7350 1 - 2 周
100 μg ￥14700 1 - 2 周
> 100 μg   询价  

* Please select Quantity before adding items.

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描述

EDNRB/Endothelin R Type B Protein, a non-specific receptor for endothelin 1, 2, and 3, activates a phosphatidylinositol-calcium second messenger system through G proteins. This mechanism underscores its pivotal role in mediating cellular responses initiated by endothelin neuropeptides, emphasizing the versatility of EDNRB in transducing signals for various physiological processes. EDNRB/Endothelin R Type B Protein-VLP, Human (HEK293, GFP) is the recombinant human-derived EDNRB/Endothelin R Type B protein, expressed by HEK293, with GFP labeled tag.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705435.
研究背景

EDNRB/内皮素 R B 型蛋白是内皮素 1、2 和 3 的非特异性受体，通过与 G 蛋白结合来激活磷脂酰肌醇-钙第二信使系统。这种分子相互作用机制强调了其在介导内皮素神经肽引发的细胞反应中的关键作用，强调了 EDNRB 在转导有助于各种生理过程的信号方面的多功能性。
种属

Human
表达系统

HEK293
标签

GFP
蛋白编号

P24530 (E27-S442)
基因 ID

1910
蛋白结构
N-term
EDNRB (E27-S442)
Accession # P24530
C-term
同用名
EDNRB; Endothelin receptor non-selective type; endothelin receptor type B; ETRB
性状

溶液.
组分

Supplied as 0.2 μm filtered solution of PBS, Arginine, pH7.4 with trehalose as protectant.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料
产品使用指南 (1213 KB)

EDNRB/Endothelin R Type B Protein-VLP, Human (HEK293, GFP) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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