Endo S2 蛋白, S. pyogenes (His)

Endo S2, secreted by Streptococcus pyogenes, deglycosylates the conserved N-linked glycan at Asn297 on IgG Fc to eliminate its effector functions and evade the immune system. EndoS2 specifically hydrolyzes diverse glycans on the Fc region of IgG antibodies using a mechanism that relies on its glycoside hydrolase domain and a coevolved carbohydrate-binding module^[1][2]. Endo S2 Protein, S. pyogenes (His) is the recombinant Endo S2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Endo S2 Protein, S. pyogenes (His) is 807 a.a., with molecular weight of ~95 kDa.

EndoS2 具有更广泛的底物聚糖特异性，不仅可以水解双触角 CT N-聚糖，还可以水解 IgG 上的高甘露糖 (HM)、杂合和二等分 CT N-聚糖^[1]。
内切-β-N-乙酰氨基葡萄糖苷酶 (ENGases，EC 3.2.1.96) 是一类酶，可以水解裂解 N- 内核区域的 N,N'-二乙酰壳二糖部分内的 β-1,4 糖苷键。聚糖并从其相关蛋白中释放聚糖链，从而留下一个单位的 N-乙酰氨基葡萄糖 (GlcNAc)，无论有或没有核心岩藻糖，都与天冬酰胺残基连接^[2]。

Measured by its ability to digest Cy5-Labeled Glycan G2>50% of Cy5-Labeled Glycan G2 (0.2 pmol) is digested by 0.2 μg of rSp. Endo-S2.

Others

E. coli

N-6*His

ACI61688.1 (E37-D843)

/

EEKTVQTGKTDQQVGAKLVQEIREGKRGPLYAGYFRTWHDRASTGIDGKQQHPENTMAEVPKEVDILFVFHDHTASDSPFWSELKDSYVHKLHQQGTALVQTIGVNELNGRTGLSKDYPDTPEGNKALAAAIVKAFVTDRGVDGLDIDIEHEFTNKRTPEEDARALNVFKEIAQLIGKNGSDKSKLLIMDTTLSVENNPIFKGIAEDLDYLLRQYYGSQGGEAEVDTINSDWNQYQNYIDASQFMIGFSFFEESASKGNLWFDVNEYDPNNPEKGKDIEGTRAKKYAEWQPSTGGLKAGIFSYAIDRDGVAHVPSTYKNRTSTNLQRHEVDNISHTDYTVSRKLKTLMTEDKRYDVIDQKDIPDPALREQIIQQVGQYKGDLERYNKTLVLTGDKIQNLKGLEKLSKLQKLELRQLSNVKEITPELLPESMKKDAELVMVGMTGLEKLNLSGLNRQTLDGIDVNSITHLTSFDISHNSLDLSEKSEDRKLLMTLMEQVSNHQKITVKNTAFENQKPKGYYPQTYDTKEGHYDVDNAEHDILTDFVFGTVTKRNTFIGDEEAFAIYKEGAVDGRQYVSKDYTYEAFRKDYKGYKVHLTASNLGETVTSKVTATTDETYLVDVSDGEKVVHHMKLNIGSGAIMMENLAKGAKVIGTSGDFEQAKKIFDGEKSDRFFTWGQTNWIAFDLGEINLAKEWRLFNAETNTEIKTDSSLNVAKGRLQILKDTTIDLEKMDIKNRKEYLSNDENWTDVAQMDDAKAIFNSKLSNVLSRYWRFCVDGGASSYYPQYTELQILGQRLSNDVANTLKD

Approximately 95 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.