1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators Biotinylated Proteins
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA2
  6. EphA2 蛋白, Human (Biotinylated, HEK293, His-Avi)

EphA2 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P72992
COA 产品使用指南

EphA2 蛋白是肝配蛋白受体亚家族的重要成员,介导发育事件,特别是在神经系统中。它具有单个激酶结构域和结合肝配蛋白-A 配体的胞外区域。EphA2 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 EphA2 蛋白,由 HEK293 表达,带有 C-6*His, C-Avi 标签。EphA2 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 511 个氨基酸,分子量约为 ~59.4 kDa。

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规格 价格 是否有货 数量
20 μg ¥2860 In-stock
100 μg ¥8000 In-stock
> 100 μg   询价  

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描述

The EphA2 protein is an important member of the ephrin receptor subfamily that mediates developmental events, particularly in the nervous system. It has a single kinase domain and an extracellular region that binds ephrin-A ligands. EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-6*His, C-Avi labeled tag. The total length of EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is 511 a.a., with molecular weight of ~59.4 kDa.

研究背景

EphA2 蛋白是蛋白酪氨酸激酶家族中肝配蛋白受体亚家族的成员,在介导发育事件中发挥着至关重要的作用,特别是在神经系统中。EPH 受体具有单个激酶结构域和具有富含 Cys 结构域和 2 个纤连蛋白 III 型重复的胞外区域,参与结合肝配蛋白 A 和肝配蛋白 B 配体。EphA2 特异性结合肝配蛋白-A 配体,并在多种组织中广泛表达,包括食道 (RPKM 37.8)、膀胱 (RPKM 18.3) 和 21 个其他组织。此外,该基因的突变与某些遗传相关的白内障疾病有关,突出了其在发育过程和眼部健康中的重要性。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized EFNA1-hFc at 10 μg/mL (100 μL/well) can bind Biotinylated EphA2 Protein, Human (HEK293, His-Avi) and the EC50 is 20-40 ng/mL.

种属

Human

表达系统

HEK293

标签

C-6*His;C-Avi

蛋白编号

NP_004422.2 (A24-N534)

基因 ID
蛋白结构
N-term
EphA2 (A24-N534)
Accession # NP_004422.2
Avi-His
C-term
同用名
Ephrin type-A receptor 2; Epithelial cell kinase; EPHA2; ECK
氨基酸序列

LKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI

分子量

Approximately 59.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EphA2 Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P72992
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