ER alpha/ESR1 蛋白, Human (His)

The ER α/ESR1 protein is a nuclear receptor that plays a critical regulatory role in gene expression, affecting cell proliferation and differentiation. Ligand-dependent transactivation involves binding of homodimers to estrogen response elements or association with transcription factors. ER alpha/ESR1 Protein, Human (His) is the recombinant human-derived ER alpha/ESR1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ER alpha/ESR1 Protein, Human (His) is 116 a.a., with molecular weight of ~14 kDa.

ER α/ESR1 蛋白是一种核激素受体，在真核基因表达调节中发挥着关键作用，影响靶组织中的细胞增殖和分化。配体依赖性核反式激活涉及同二聚体直接结合回文雌激素反应元件 (ERE) 序列或与 DNA 结合转录因子（如 AP-1/c-Jun、c-Fos、ATF-2、Sp1 和 Sp3）关联，促进 ERE - 独立的信号发送。配体结合后，ER α 会发生构象变化，从而能够随后通过 LXXLL 基序与多蛋白共激活剂复合物结合。ER α 和 NF-kappa-B 之间以细胞类型特异性方式发生相互反式抑制，导致 NF-kappa-B DNA 结合活性降低并抑制 NF-kappa-B 介导的转录。ER α 被招募到 NF-kappa-B 反应元件中，取代辅助调节因子并与 NF-kappa-B 协同介导转录激活。此外，ER α 通过各种激酶级联参与膜启动的雌激素信号传导，并且对于 MTA1 介导的 BRCA1 和 BCAS3 转录调节至关重要。此外，ER α 还参与 NOS3 的激活和内皮一氧化氮的产生。缺乏特定功能域的异构体被认为可以通过竞争性配体或 DNA 结合以及与全长受体的异二聚化来调节转录活性。此外，ER α 与 ERE 结合并抑制异构体 1。

Data is not available.

Human

E. coli

N-6*His

P03372 (M1-Q116)

2099  [NCBI]

MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQ

Approximately 14 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Glycine-HCl, 8% Sucrose, 0.05% Tween 80, pH 3.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.