FCRN-B2M 蛋白, Human (HEK293, His)

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-6*His labeled tag. FCRN-B2M Protein, Human (HEK293, His), has molecular weight of ~32 & 12 kDa, respectively.

IgG receptor FcRn large subunit p51 是一种与 MHC I 类分子结构相似的 IgG Fc 受体，也与 β-2微球蛋白结合。在啮齿类动物中，FcRn 最初被认为是通过母乳将母体免疫球蛋白 G (IgG) 从母体转运到新生后代的受体，因此被称为新生 Fc 受体。FcRn 也被证明在调节 IgG 和血清白蛋白转换中发挥作用，新生儿 Fc 受体表达受促炎细胞因子 TNF 上调，IFN-γ 下调。在内皮细胞和造血细胞的酸性内体中，单体 IgG 与 FcRn 结合，将 IgG 循环到细胞表面，并在那里释放到循环中，除 IgG 外，还调节循环蛋白白蛋白/ALB 的稳态。FCGRT 在膀胱癌中表达上调可作为不良预后相关的关键中性粒细胞基因。FCGRT 过表达还与 PD-L1 表达降低和肿瘤突变负荷 (TMB) 水平降低相关。

Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2.00 µg/mL (100 µL/well), can bind  Biotinylated Human lgG1. The ED₅₀ for this effect is 81.59 ng/mL.

Human

HEK293

C-6*His

AAF72596 (A24-S297)&P61769 (I21-M119)

2217  [NCBI]&567  [NCBI]

A1:
AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS
A2:
IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Approximately 32 & 12 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES,150 mM Nacl, 0.02% Tween20, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.