1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-8
  6. FGF-8b 蛋白, Human/Mouse

FGF-8b 蛋白, Human/Mouse

目录号: HY-P70533
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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥450 In-stock
10 μg ¥1200 In-stock
50 μg ¥3600 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

1.Measured in a cell proliferation assay using BALB/c 3T3 cells and the special bioactivity is 12-40.58 ng/mL.
2.Measured in a cell proliferation assay using NIH/3T3 mouse fibroblast cells. The ED50 for this effect is typically 51.21 ng/mL in the presence of 1 µg/mL heparin,corresponding to a specific activity is 1.95×104 U/mg.

  • Measured in a cell proliferation assay using NIH/3T3 mouse fibroblast cells. The ED50 for this effect is typically 51.21 ng/mL in the presence of 1 µg/mL heparin,corresponding to a specific activity is 1.95×10^4 U/mg.
种属

Mouse; Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P55075-3/P37237-2 (Q23-R215)

基因 ID

2253  [NCBI]/14179  [NCBI]

蛋白结构
N-term
FGF-8b (Q23-R215)
Accession # P55075-3
C-term
同用名
Fibroblast growth factor 8; Androgen-induced growth factor; Heparin-binding growth factor 8; AIGF; HBGF-8; FGF-8B
氨基酸序列

QVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR

分子量

21-24 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM PB,300 mM NaCl,2% Sucrose, 0.02% Tween 80,pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

FGF-8b Protein, Human/Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGF-8b Protein, Human/Mouse
目录号:
HY-P70533
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