FUOM/Fucose Mutarotase 蛋白, Human (His)

The FUOM/fucose mutarotase protein has a crucial interconversion effect on α- and β-L-fucose, with β-L-fucose being dominant (70.5%). The β-form undergoes metabolic processes via the salvage pathway, producing GDP-L-fucose that is transported to the endoplasmic reticulum. FUOM/Fucose Mutarotase Protein, Human (His) is the recombinant human-derived FUOM/Fucose Mutarotase protein, expressed by E. coli , with N-His labeled tag. The total length of FUOM/Fucose Mutarotase Protein, Human (His) is 154 a.a., with molecular weight of ~19 kDa.

FUOM/岩藻糖变旋酶蛋白在 α-L-岩藻糖和 β-L-岩藻糖之间的相互转化中发挥着至关重要的作用，其中 L-岩藻糖（一种 6-脱氧-L-半乳糖）以 α-L-岩藻糖 (29.5%) 和 β 的形式共存-L-岩藻糖（70.5%）。值得注意的是，β-形式通过挽救途径经历代谢过程。GDP-L-岩藻糖通过从头途径或挽救途径产生，被转运至内质网。在那里，它充当岩藻糖基转移酶催化的 N-和 O-糖基化的底物。人们认为在细胞表面表达或在生物体液中分泌的岩藻糖基化结构在细胞间粘附和识别过程中发挥关键作用。FUOM 促进的动态相互转化凸显了其在调节可用 L-岩藻糖库、影响对各种细胞功能至关重要的必需糖基化事件方面的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

A2VDF0 (M1-L154)

282969  [NCBI]

Approximately 19 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.