1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Furin/PCSK3 蛋白, Human (HEK293, His)

Furin/PCSK3 蛋白, Human (HEK293, His)

目录号: HY-P78747
COA 产品使用指南

Furin/PCSK3 蛋白是组成型分泌途径中普遍存在的内切蛋白酶,可有效切割 RX(K/R)R 共有基序。它的多功能性在处理 TGFB1(TGF-β-1 激活所必需)和将脑钠尿因子激素原转化为活性 BNP(1-32) 方面表现得很明显。Furin/PCSK3 蛋白, Human (HEK293, His) 是重组的 弗林蛋白酶/PCSK3 蛋白,由 HEK293 表达,带有 C-His, C-6*His 标签。Furin/PCSK3 蛋白, Human (HEK293, His) 全长 548 个氨基酸,分子量为 60-75 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Furin/PCSK3 protein is a ubiquitous endoprotease in the constitutive secretory pathway that efficiently cleaves the RX(K/R)R consensus motif. Its versatility is evident in processing TGFB1 (essential for TGF-β-1 activation) and converting the brain natriuretic factor prohormone into active BNP(1-32). Furin/PCSK3 Protein, Human (HEK293, His) is the recombinant human-derived Furin/PCSK3 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of Furin/PCSK3 Protein, Human (HEK293, His) is 548 a.a., with molecular weight of 60-75 kDa.

研究背景

Furin/PCSK3 蛋白是一种在组成型分泌途径中普遍存在的内切蛋白酶,表现出在 RX(K/R)R 共有基序上切割的卓越能力。这种酶的多功能性体现在它参与 TGFB1 的加工,这是激活 TGF-β-1 的关键步骤。Furin/PCSK3 在非功能性脑钠尿因子激素原转化为其活性激素 BNP(1-32) 的过程中也发挥着关键作用。此外,通过介导 V-ATP 酶的辅助亚基 ATP6AP1/Ac45 的加工,Furin/PCSK3 有助于调节朗格汉斯细胞胰岛中致密核心分泌颗粒内的酸性环境。值得注意的是,在微生物感染的情况下,这种蛋白酶参与白喉毒素 DT 的裂解和激活。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specific activity is 191.99 pmol/min/µg, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-His;C-6*His

蛋白编号

P09958 (Q27-A574)

基因 ID
蛋白结构
N-term
Furin (Q27-A574)
Accession # P09958
6*His
C-term
中文名
重组人PCSK3 蛋白
同用名
FURIN; FUR; PACE; PCSK3; SPC1
氨基酸序列

QKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTA

分子量

60-75 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH8.0, 20% Glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Furin/PCSK3 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Furin/PCSK3 Protein, Human (HEK293, His)
目录号:
HY-P78747
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