1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FUT8 蛋白, Human (sf9, His)

FUT8 蛋白, Human (sf9, His)

目录号: HY-P75181
COA 产品使用指南

FUT8 蛋白在糖基化过程中充当催化剂,专门将岩藻糖以 α1-6 连接形式添加到位于 N-聚糖肽链附近的第一个 GlcNAc 残基上。这种酶活性在糖蛋白修饰中发挥着至关重要的作用,有助于 N-聚糖的结构多样性和功能特性。通过促进岩藻糖以特定的连接方式添加,FUT8 参与复杂的糖基化分子景观,影响各种细胞过程和涉及糖蛋白的相互作用。FUT8 蛋白, Human (sf9, His) 是重组的 FUT8 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。FUT8 蛋白, Human (sf9, His) 全长 508 个氨基酸,分子量约为 ~55 kDa。

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20 μg ¥4000 询价
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描述

FUT8 protein serves as a catalyst in the glycosylation process, specifically adding fucose in an alpha 1-6 linkage to the first GlcNAc residue located adjacent to peptide chains in N-glycans. This enzymatic activity plays a crucial role in modifying glycoproteins, contributing to the structural diversity and functional properties of N-glycans. By facilitating the addition of fucose in a specific linkage, FUT8 participates in the intricate molecular landscape of glycosylation, influencing various cellular processes and interactions involving glycoproteins. FUT8 Protein, Human (sf9, His) is the recombinant human-derived FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Human (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

研究背景

FUT8 蛋白在糖基化过程中充当催化剂,专门将岩藻糖以 α1-6 连接形式添加到位于 N-聚糖肽链附近的第一个 GlcNAc 残基上。这种酶活性在糖蛋白修饰中发挥着至关重要的作用,有助于 N-聚糖的结构多样性和功能特性。通过促进岩藻糖以特定的连接方式添加,FUT8 参与复杂的糖基化分子景观,影响各种细胞过程和涉及糖蛋白的相互作用。

生物活性

Measured by its ability to hydrolyze the donor substrate GDP fucose and the specific activity is >0.75 pmoles/min/μg.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q9BYC5 (R68-K575)

基因 ID
蛋白结构
N-term
FUT8 (R68-K575)
Accession # Q9BYC5
His
C-term
同用名
Alpha-(1,6)-fucosyltransferase; Alpha1-6FucT; FUT8; Fucosyltransferase 8
分子量

Approximately 55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

FUT8 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FUT8 Protein, Human (sf9, His)
目录号:
HY-P75181
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