1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. G6PD 蛋白, Human (HEK293, His)

G6PD 蛋白, Human (HEK293, His)

目录号: HY-P70283
COA 产品使用指南

G6PD 蛋白通过催化氧化戊糖磷酸途径的限速步骤在细胞代谢中发挥关键作用,这对于糖酵解过程中碳水化合物的异化至关重要。该酶的主要功能是以 NADPH 形式提供还原力并生成磷酸戊糖,支持脂肪酸和核酸合成等基本过程。G6PD 蛋白, Human (HEK293, His) 是重组的 G6PD 蛋白,由 HEK293 表达,带有 C-6*His 标签。G6PD 蛋白, Human (HEK293, His) 全长 514 个氨基酸,分子量为 55-62 kDa。

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规格 价格 是否有货 数量
10 μg ¥1702 In-stock
50 μg ¥5100 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

G6PD proteins play a key role in cellular metabolism by catalyzing the rate-limiting step of the oxidative pentose phosphate pathway, which is essential for carbohydrate dissimilation during glycolysis. The primary function of this enzyme is to provide reducing power in the form of NADPH and generate pentose phosphate, supporting essential processes such as fatty acid and nucleic acid synthesis. G6PD Protein, Human (HEK293, His) is the recombinant human-derived G6PD protein, expressed by HEK293 , with C-6*His labeled tag. The total length of G6PD Protein, Human (HEK293, His) is 514 a.a., with molecular weight of 55-62 kDa.

研究背景

G6PD(6-磷酸葡萄糖脱氢酶)蛋白是一种关键酶,可催化氧化戊糖磷酸途径中的限速步骤,为碳水化合物异化和糖酵解提供了另一种途径。G6PD 的主要功能是以 NADPH 和磷酸戊糖的形式提供必需的还原能力,它们在脂肪酸和核酸的合成中发挥着关键作用。NADPH 对于维持细胞氧化还原平衡至关重要,并作为各种生物合成过程的辅助因子,而戊糖磷酸有助于核苷酸和其他细胞成分的合成。G6PD 在细胞代谢中的核心作用强调了其在支持细胞生长和增殖所必需的合成代谢途径方面的重要性。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P11413-1 (A2-L515)

基因 ID
蛋白结构
N-term
G6PD (A2-L515)
Accession # P11413
6*His
C-term
同用名
rHuGlucose-6-phosphate 1-dehydrogenase/G6PD, His; Glucose-6-Phosphate 1-Dehydrogenase; G6PD
氨基酸序列

AEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

分子量

55-62 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20mM Citrate, 15% Trehalose, 150mM NaCl, 0.05% Tween 80, pH5.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

G6PD Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
G6PD Protein, Human (HEK293, His)
目录号:
HY-P70283
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