GARS 蛋白, Human (sf9, His)

The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.

GARS 是一种关键酶，通过形成氨酰基腺苷酸中间体 (Gly-AMP)，促进甘氨酸与其相应 tRNA 3' 端的 ATP 依赖性连接。这个过程对于蛋白质合成和维持遗传密码的保真度至关重要。此外，GARS 具有独特的能力，可以通过直接缩合两个 ATP 分子来生成四磷酸二腺苷 (Ap4A)，这是一种参与多种细胞调节途径的通用信号分子。这种双重酶活性强调了 GARS 在蛋白质合成等基本细胞过程中的多方面作用，以及通过 Ap4A 调节信号级联反应，表明其在 Ap4A 稳态中的潜在重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P41250-1/NP_002038.2 (M1-E739)

2617  [NCBI]

Full Length of Isoform-1

Approximately 85.5 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, pH 7.5, 150 mM Nacl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.