1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. GARS 蛋白, Human (sf9, His)

GARS 蛋白, Human (sf9, His)

目录号: HY-P74128
产品使用指南

GARS 是一种关键酶,通过形成氨酰基腺苷酸中间体 (Gly-AMP),促进甘氨酸与其相应 tRNA 3' 端的 ATP 依赖性连接。这个过程对于蛋白质合成和维持遗传密码的保真度至关重要。此外,GARS 具有独特的能力,可以通过直接缩合两个 ATP 分子来生成四磷酸二腺苷 (Ap4A),这是一种参与多种细胞调节途径的通用信号分子。这种双重酶活性强调了 GARS 在蛋白质合成等基本细胞过程中的多方面作用,以及通过 Ap4A 调节信号级联反应,表明其在 Ap4A 稳态中的潜在重要性。GARS 蛋白, Human (sf9, His) 是重组的 GARS 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。GARS 蛋白, Human (sf9, His) 全长 739 个氨基酸,分子量约为 ~85.5 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

GARS, a pivotal enzyme, facilitates the ATP-dependent ligation of glycine to the 3'-end of its corresponding tRNA through the formation of an aminoacyl-adenylate intermediate (Gly-AMP). This process is crucial for protein synthesis and maintaining the fidelity of the genetic code. Additionally, GARS exhibits a unique capability to generate diadenosine tetraphosphate (Ap4A), a universal signaling molecule involved in diverse cell regulation pathways, by directly condensing two ATP molecules. This dual enzymatic activity underscores the multifaceted role of GARS in both fundamental cellular processes, such as protein synthesis, and the modulation of signaling cascades through Ap4A, suggesting its potential significance in Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of GARS Protein, Human (sf9, His) is 739 a.a., with molecular weight of ~85.5 kDa.

研究背景

GARS 是一种关键酶,通过形成氨酰基腺苷酸中间体 (Gly-AMP),促进甘氨酸与其相应 tRNA 3' 端的 ATP 依赖性连接。这个过程对于蛋白质合成和维持遗传密码的保真度至关重要。此外,GARS 具有独特的能力,可以通过直接缩合两个 ATP 分子来生成四磷酸二腺苷 (Ap4A),这是一种参与多种细胞调节途径的通用信号分子。这种双重酶活性强调了 GARS 在蛋白质合成等基本细胞过程中的多方面作用,以及通过 Ap4A 调节信号级联反应,表明其在 Ap4A 稳态中的潜在重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P41250 (M1-E739)

基因 ID
蛋白结构
N-term
His
GARS (M1-E739)
Accession # P41250
C-term
同用名
Glycine--tRNA ligase; GlyRS; GARS1; GARS
分子量

Approximately 85.5 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GARS Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
GARS Protein, Human (sf9, His)
目录号:
HY-P74128
需求量: