1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GhrA 蛋白, E.coli (His-SUMO)

GhrA 蛋白, E.coli (His-SUMO)

目录号: HY-P71523
产品使用指南

GhrA 是一种参与细胞代谢的酶,在催化 NADPH 依赖性乙醛酸还原为乙醇酸以及羟基丙酮酸还原为甘油酸方面发挥着至关重要的作用。这种酶活性有助于重要代谢物的转化,并有助于与乙醛酸解毒和维持细胞稳态相关的代谢途径。GhrA 蛋白, E.coli (His-SUMO) 是重组的 GhrA 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。GhrA 蛋白, E.coli (His-SUMO) 全长 312 个氨基酸,分子量约为 ~51.4 kDa。

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描述

GhrA is an enzyme involved in cellular metabolism and plays a crucial role in catalyzing the NADPH-dependent reduction of glyoxylate to glycolate and hydroxypyruvate to glycerate. This enzymatic activity contributes to the conversion of important metabolites and contributes to metabolic pathways related to glyoxylate detoxification and maintenance of cellular homeostasis. GhrA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived GhrA protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of GhrA Protein, E.coli (His-SUMO) is 312 a.a., with molecular weight of ~51.4 kDa.

研究背景

GhrA 是一种参与细胞代谢的酶,它在催化 NADPH 依赖性的乙醛酸还原为乙醇酸以及羟基丙酮酸还原为甘油酸方面发挥着至关重要的作用。这种酶活性有助于重要代谢物的转化,有助于与乙醛酸解毒和维持细胞稳态相关的代谢途径。

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

B7LFE3 (1M-312Y)

基因 ID

/

蛋白结构
N-term
6*His-SUMO
GhrA (1M-312Y)
Accession # B7LFE3
C-term
同用名
ghrA; EC55989_1146Glyoxylate/hydroxypyruvate reductase A; EC 1.1.1.79; EC 1.1.1.81; 2-ketoacid reductase
氨基酸序列

MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALVWHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRFPLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPDGAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPENPLWQHPRVTITPHVAAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY

分子量

Approximately 51.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GhrA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GhrA Protein, E.coli (His-SUMO)
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HY-P71523
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