Glypican-1/GPC1 蛋白, Mouse (HEK293, His)

Glypican-1 is a cell surface proteoglycan containing heparan sulfate that binds alpha-4 (V) collagen and aids in Schwann cell myelination. Glypican-1/GPC1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Glypican-1/GPC1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Glypican-1/GPC1 Protein, Mouse (HEK293, His) is 506 a.a., with molecular weight of ~60 kDa.

Glypican-1 (GPC1) 蛋白是一种细胞表面蛋白聚糖，其特征在于存在硫酸乙酰肝素。它通过硫酸乙酰肝素侧链与 alpha-4 (V) 胶原蛋白结合，在雪旺细胞髓鞘形成中发挥作用。此外，GPC1 可以充当催化剂，通过其硫酸乙酰肝素侧链与两种形式的 PRPN 结合，提高朊病毒蛋白 PRPN(C) 向 PRNP(Sc) 的转化率，促进它们的相互作用并将其靶向脂筏。GPC1 对于骨骼肌的正常分化至关重要，因为它将 FGF2 隔离在脂筏中，防止其与受体 (FGFR) 结合并抑制 FGF 介导的信号传导。此外，GPC1 具有结合 Cu(2+) 或 Zn(2+) 离子的能力。

2 μg/mL (100 μL/well) of immobilized Human FGFb can bind Mouse GPC1-His with an ED₅₀ value of 0.4-2 μg/mL.

Mouse

HEK293

C-6*His

Q9QZF2 (D24-S529)

14733  [NCBI]

DPASKSRSCSEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANHSRMELESALHDSSRALQATLATQLHGIDDHFQRLLNDSERTLQEAFPGAFGDLYTQNTRAFRDLYAELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLPDDYLDCLGKQAEALRPFGDAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLAPECSRAIMKLVYCAHCRGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGPSGAESVIGGVHVWLAEAINALQDNKDTLTAKVIQACGNPKVNPHGSGPEEKRRRGKLALQEKPSTGTLEKLVSEAKAQLRDIQDFWISLPGTLCSEKMAMSPASDDRCWNGISKGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRGAYGGNDVDFQDASDDGSGSGSGGGCPDDTCGRRVSKKSSSSRTPLTHALPGLSEQEGQKTS

Approximately 60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.