Granzyme H/GZMH 蛋白, Human (HEK293, His)

The Granzyme H/GZMH protein, encoded by this gene, belongs to the peptidase S1 family. It is expressed in NK cells and plays a role in the cytotoxic arm of the innate immune response. It induces target cell death and cleaves substrates in infected cells. It is located on chromosome 14 and is broadly expressed, including in the spleen, bone marrow, and 21 other tissues. Granzyme H/GZMH Protein, Human (HEK293, His) is the recombinant human-derived Granzyme H/GZMH, expressed by HEK293, with C-His labeled tag. The total length of Granzyme H/GZMH Protein, Human (HEK293, His) is 228 a.a..

粒酶 H/GZMH 蛋白是丝氨酸蛋白酶肽酶 S1 家族的成员，由该基因编码。通过选择性剪接，产生了多种转录变体，其中至少一种编码前原蛋白，该前蛋白经过蛋白水解加工产生胰凝乳蛋白酶样蛋白酶。该蛋白在免疫系统的 NK（自然杀伤）细胞中持续表达，据信在先天免疫反应的细胞毒性臂中发挥作用。它诱导靶细胞死亡并直接裂解病原体感染细胞中的底物。该基因位于 14 号染色体上的基因簇中，旁边是粒酶亚家族的另一个成员。粒酶 H/GZMH 蛋白表现出广泛的表达，在脾脏、骨髓和 21 种其他组织等组织中具有显着水平。

Measured by its ability to cleave a peptide substrate, 100 µM Suc-FLF-SBzl, in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). that incubate at room temperaturein kinetic mode for 5 minutes.The specific activity is >5,000 pmol/min/µg. (Activation description: The proenzyme needs to be activated by Mouse Cathepsin C (HY-P704018) for an activated form.)

Human

HEK293

C-6*His

NP_219491.1 (E19-L246)

2999  [NCBI]

Full Length of Mature Protein

Approximately 35-40 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 8.0, 0.1% Brij-35.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.