1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, Influenza A H5N1 (sf9, His)

HA/Hemagglutinin 蛋白, Influenza A H5N1 (sf9, His)

目录号: HY-P73238
产品使用指南

HA/Hemagglutinin 蛋白是一种病毒膜蛋白,负责流感病毒进入宿主细胞的最初步骤。它介导病毒颗粒与宿主细胞膜的结合,并催化病毒膜与宿主膜的融合。HA 在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。HA/Hemagglutinin 蛋白, Influenza A H5N1 (sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。HA/Hemagglutinin 蛋白, Influenza A H5N1 (sf9, His) 全长 531 个氨基酸,分子量约为 ~60.1 kDa。

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描述

HA/Hemagglutinin Protein is a viral membrane protein responsible for the initial steps of the entry of influenza virus into the host cell. It mediates binding of the virus particle to the host-cell membrane and catalyzes fusion of the viral membrane with that of the host. HA is pivotal in determining virus host range and virulence. HA/Hemagglutinin Protein, Influenza A H5N1 (sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, Influenza A H5N1 (sf9, His) is 531 a.a., with molecular weight of ~60.1 kDa.

研究背景

血凝素蛋白 (HA) 是一种 I 类病毒融合蛋白,与细胞表面上含唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着通过网格蛋白依赖性内吞作用诱导约三分之二的病毒颗粒内化,并通过网格蛋白和小窝蛋白独立途径诱导约三分之一的病毒颗粒内化。HA 在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。通过介导内吞病毒颗粒膜与内体膜的融合,HA 使病毒能够渗透到细胞质中。在内体的低pH环境下,HA2发生不可逆的构象变化,释放融合疏水肽并形成有能力的融合孔。HA 作为由二硫键连接的 HA1-HA2 亚基组成的同源三聚体存在,并与人 CACNA1C 相互作用[1][2][3]

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

AAT76166.1 (M1-Q531)

基因 ID

/

蛋白结构
N-term
H5N1 HA (M1-Q531)
Accession # AAT76166.1
His
C-term
同用名
HA; Hemagglutinin
分子量

Approximately 60.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, Influenza A H5N1 (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, Influenza A H5N1 (sf9, His)
目录号:
HY-P73238
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