1. 重组蛋白
  2. Viral Proteins
  3. HAdV-E fiber 蛋白 (sf9, His)

HAdV-E fiber 蛋白 (sf9, His)

目录号: HY-P76391
产品使用指南

Human mastadenoviruses (HAdVs) 是无包膜双链 DNA 病毒。Human adenovirus type 4 (HAdV-E4) 是迄今为止从人类中分离出的 Human adenovirus type 4 (HAdV-E4) 物种中唯一的类型。HAdV-E4 感染与急性呼吸道疾病有关。HAdV-E fiber 蛋白 (sf9, His) 是重组的 HAdV-E 纤维蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。HAdV-E fiber 蛋白 (sf9, His) 全长 425 个氨基酸,分子量约为 ~46.56 KDa。

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描述

Human mastadenoviruses (HAdVs) are non-enveloped, double-stranded DNA viruses. Human adenovirus type 4 (HAdV-E4) is the only type in species Human mastadenovirus E (HAdV-E) thus far isolated from humans. HAdV-E4 infection is associated with acute respiratory disease[1][2]. HAdV-E fiber Protein (sf9, His) is the recombinant Virus-derived HAdV-E fiber protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HAdV-E fiber Protein (sf9, His) is 425 a.a., with molecular weight of ~46.56 KDa.

研究背景

目前已识别的超过 89 种人类腺病毒 (HAdV) 基因型根据其遗传特征分为七种,称为人类乳腺病毒 A 至 G (HAdV-A 至 HAdV-G)。主要抗原六邻体上的属特异性表位常用于腺病毒的免疫学常规诊断。外部病毒衣壳六邻体和五邻体的成分还具有中和抗体的表位。该纤维包含主要具有属特异性表位的轴和主要具有物种特异性表位的旋钮,应该适用于属特异性而且也适用于物种特异性免疫学诊断,就像已经用于通过血凝抑制对腺病毒进行血清分型一样 基于纤维决定因素的测定[1][2]

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

YP_068048 (M1-Q425)

基因 ID

2958476  [NCBI]

蛋白结构
N-term
Fiber (M1-Q425)
Accession # YP_068048
His
C-term
同用名
Human mastadenovirus E (HAdV-E) fiber Protein (His)
分子量

Approximately 46.56 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HAdV-E fiber Protein (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HAdV-E fiber Protein (sf9, His)
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