ICAM-1/CD54 蛋白, Human (HEK293, Fc)

The ICAM-1/CD54 protein plays a crucial role in cell interactions, acting as a ligand for the leukocyte adhesion protein LFA-1. It promotes leukocyte transendothelial migration by promoting the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. ICAM-1/CD54 Protein, Human (HEK293, Fc) is the recombinant human-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ICAM-1/CD54 Protein, Human (HEK293, Fc) is 453 a.a., with molecular weight of ~95-135 kDa.

ICAM-1/CD54 蛋白在细胞相互作用中发挥着重要作用，充当白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的配体。特别值得注意的是它在白细胞跨内皮迁移过程中的作用，其中与 ICAM-1 的结合通过激活 ARHGEF26/SGEF 和 RHOG 促进内皮顶杯的组装。在微生物感染的情况下，ICAM-1 还充当主要受体组鼻病毒 AB 衣壳蛋白的受体，突显其在免疫反应和病原体识别中的多功能参与。这种双重功能强调了 ICAM-1 在介导对免疫调节和宿主防御至关重要的多种细胞过程中的重要性。

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5×10⁴ cells/well are added to ICAM-1 coated plates (12.5 μg/mL,100 μL/well) in the presence of 20 ng/mL PMA, approximately 79.7% will adhere after 1 hour at 37°C.

Human

HEK293

C-hFc

P05362/NP_000192.2 (Q28-E480)

3383  [NCBI]

QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE

Approximately 95-135 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.