IFN-gamma 蛋白, Porcine

IFN-gamma (interferon-gamma) is a type II interferon produced by immune cells such as T cells and NK cells. It plays a key role in antibacterial, antiviral and anti-tumor responses by activating effector immune cells and enhancing antigen presentation. effect. Its main signaling pathway involves the JAK-STAT pathway that interacts with its receptor IFNGR1, affecting gene regulation. IFN-gamma Protein, Porcine is the recombinant Porcine-derived IFN-gamma protein, expressed by E. coli , with tag free. The total length of IFN-gamma Protein, Porcine is 146 a.a., with molecular weight of ~17.3 kDa.

IFN-gamma（干扰素-gamma）是一种由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥关键作用。其主要信号通路涉及与其受体 IFNGR1 相互作用的 JAK-STAT 通路，影响基因调控。IFN-γ 结合后，IFNGR1 胞内结构域打开，促进下游信号成分（包括 JAK2、JAK1 和 STAT1）的关联。该级联导致 STAT1 激活、核转位以及 IFN-γ 调节基因的后续转录，其中许多是像 IRF1 一样的转录因子，能够驱动后续的转录波。IFN-γ 通过诱导免疫蛋白酶体亚基替换催化蛋白酶体亚基，从而促进 I 类抗原呈递途径，从而提高 I 类 MHC 负载的肽的数量、质量和库。它还通过诱导激活剂 PA28 的表达来提高肽生成的效率，PA28 与蛋白酶体结合并改变其蛋白水解切割偏好。此外，IFN-γ 通过促进组织蛋白酶 B/CTSB、H/CTSH 和 L/CTSL 等关键分子的表达，上调细胞表面的 MHC II 复合物。除了其直接的免疫功能外，IFN-γ还参与造血干细胞在发育过程中和稳态条件下的调节，影响其发育、静止和分化。IFN-γ 作为同二聚体存在，通过其胞外结构域与 IFNGR1 相互作用，这是促进 IFNGR1 二聚化的关键相互作用，协调其在免疫反应和造血中的多样化和关键功能。

1.Measured in an anti-viral assay using PK-15 porcine kidney epithelial cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 0.015-0.045 ng/mL.
2.Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 0.3912 ng/mL, corresponding to a specificactivity is 2.556×10⁶ Unit/mg.

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  Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED₅₀ for this effect is 0.3912 ng/mL, corresponding to a specificactivity is 2.556×10⁶ Unit/mg.

Porcine

E. coli

Tag Free

P17803 (S21-K166)

396991  [NCBI]

SYCQAPFFKEITILKDYFNASTSDVPNGGPLFLEILKNWKEESDKKIIQSQIVSFYFKFFEIFKDNQAIQRSMDVIKQDMFQRFLNGSSGKLNDFEKLIKIPVDNLQIQRKAISELIKVMNDLSPRSNLRKRKRSQTMFQGQRASK

Approximately 17.3 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 50 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.