1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interferon & Receptors
  4. IFN-α/β Receptor
  5. IFNAR1
  6. IFNAR1 蛋白, Human (HEK293, His-Avi)

IFNAR1 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78457
产品使用指南

IFNAR1,IFN-α/β 受体的亚基之一,是 I 型 IFN 受体。IFNAR1 可与 IFNAR2 形成异二聚体受体。IFNAR1 通过与酪氨酸激酶 2 (Tyk2) 相互作用而介导 IFN 诱导的 STAT 信号传导。在被这些 IFN 激活后,IFNAR1 和 IFNAR2 会发生构象变化以促进下游信号事件级联反应的发生,包括 Tyk2 和 JAK1、STAT1 和 STAT2 的磷酸化。IFNAR1 蛋白, Human (HEK293, His-Avi) 是一种由 HEK293 细胞产生的重组人 IFNAR1 (K28-K436),C 端带有 His 和 Avi 标签。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

IFNAR1, one of the subunit of IFN-α/β receptor, is a type I IFN receptor. IFNAR1 forms the heterodimeric receptor with IFNAR2. IFNAR1 mediates IFN-induced STAT signaling by interacting with tyrosine kinase 2 (Tyk2)[1][2]. Upon activation by these IFNs, IFNAR1 and IFNAR2 undergo a conformational change to promote a cascade of downstream signaling events, including the phosphorylation of Tyk2 and JAK1, STAT1 and STAT2[3]. IFNAR1 Protein, Human (HEK293, His-Avi) is a recombinant human IFNAR1 (K28-K436) with C-terminal His and Avi tag, which is produced in HEK293 cells.

研究背景

IFNAR1, one of the subunit of IFN-α/β receptor, is a type I IFN receptor. IFNAR1 is expressed on peripheral blood B cells and monocytes, and mediates differentiation and activation of these cells[4].
IFNAR1 forms the heterodimeric receptor (IFN-α/β receptor) together with IFNAR2. IFNAR1 interacts with tyrosine kinase 2 (Tyk2), and the interaction is able to stabilize IFNAR1 on the plasma membrane[1]. Besides, IFNAR1 associates with TYK2 and initiates type I IFN-induced STAT signaling, but the activation needs IFNAR2 as a platform[2]. IFN-α/-β can induce association of the IFNAR1 and IFNAR2, which makes JAK1 and TYK2 form a functional signaling unit[1]. Upon activation by these IFNs, IFNAR1 and IFNAR2 undergo a conformational change to promote a cascade of downstream signaling events. The signaling events includes the phosphorylation of Tyk2 and JAK1, the signal transducers and activators of transcription STAT1 and STAT2, and the formation of the IFN-stimulated gene factor 3 (ISGF3) complex which consists of phosphorylated STAT1 and STAT2 and IRF9[3].
Human IFNAR1 consists of extracellular domain (K28-K436), helical domain (I437-A457), and cytoplasmic domain (K458-V557). The sequence of amino acids in IFNAR1 differs in different species. Human IFNAR1 shares <50% aa sequence identity with mouse.
IFNAR1 mediates IFN-induced STAT signaling by interacting with tyrosine kinase 2 (Tyk2)[1].

生物活性

Immobilized Human IFNAR1 at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-IFNAR1 Ab hFc with the EC50 of 21.2 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P17181 (K28-K436)

中文名
重组人 IFNAR1 蛋白 (HEK293, His-Avi)
同用名
AVP; IFN-alpha/beta R1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-R-1; CRF2-1; IFRC ; IFN-alpha/β R1
分子量

74-95 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFNAR1 Protein, Human (HEK293, His-Avi)
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