1. 重组蛋白
  2. Cytokines and Growth Factors
  3. IGF family
  4. IGFBP-6
  5. IGFBP-6 蛋白, Human (HEK293, His)

IGFBP-6 蛋白, Human (HEK293, His)

目录号: HY-P73141
COA 产品使用指南

IGFBP-6蛋白是重要的IGFBP成员,通过改变其与细胞表面受体的相互作用来调节胰岛素样生长因子(IGF),从而影响IGF诱导的生长反应。除了调节 IGF 信号传导外,IGFBP-6 还激活 MAPK 通路并诱导细胞迁移。IGFBP-6 蛋白, Human (HEK293, His) 是重组的 IGFBP-6 蛋白,由 HEK293 表达,带有 C-His 标签。IGFBP-6 蛋白, Human (HEK293, His) 全长 240 个氨基酸,分子量约为 ~36 kDa。

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规格 价格 是否有货 数量
10 μg ¥1950 In-stock
50 μg ¥5450 In-stock
100 μg   询价  

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描述

IGFBP-6 protein is an important IGFBP member that regulates insulin-like growth factor (IGF) by changing its interaction with cell surface receptors, thereby affecting IGF-induced growth responses. In addition to regulating IGF signaling, IGFBP-6 also activates the MAPK pathway and induces cell migration. IGFBP-6 Protein, Human (HEK293, His) is the recombinant human-derived IGFBP-6 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-6 Protein, Human (HEK293, His) is 240 a.a., with molecular weight of ~36 kDa.

研究背景

IGFBP-6 蛋白是胰岛素样生长因子结合蛋白 (IGFBP) 的成员,在调节胰岛素样生长因子 (IGF) 的半衰期和调节其对细胞培养的影响方面发挥着关键作用,表现出抑制或刺激 IGF 诱导的生长促进反应的能力。这种调节功能是通过改变 IGF 与其各自细胞表面受体的相互作用来实现的。除了调节 IGF 信号传导的作用外,IGFBP-6 还表现出其他细胞效应,例如激活 MAPK 信号传导途径和诱导细胞迁移。值得注意的是,IGFBP-6 通过其 C 端结构域与 PHB2 相互作用,突显其参与蛋白质-蛋白质相互作用,可能有助于多种细胞过程。IGFBP-6 的多方面作用强调了其在协调与生长、迁移和信号传导途径相关的复杂细胞反应中的重要性。

生物活性

1. Measured by its ability to bind human IGF1 in functional ELISA.
2. Measured by its ability to bind human IGF2 in functional ELISA.
3. Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 1-5 μg/mL in the presence of 14 ng/mL human IGFII.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P24592 (M1-G240)

基因 ID
蛋白结构
N-term
IGFBP-6 (M1-G240)
Accession # P24592
His
C-term
同用名
Insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IBP6
氨基酸序列

MTPHRLLPPLLLLLALLLAASPGGALARCPGCGQGVQAGCPGGCVEEEDGGSPAEGCAEAEGCLRREGQECGVYTPNCAPGLQCHPPKDDEAPLRALLLGRGRCLPARAPAVAEENPKESKPQAGTARPQDVNRRDQQRNPGTSTTPSQPNSAGVQDTEMGPCRRHLDSVLQQLQTEVYRGAQTLYVPNCDHRGFYRKRQCRSSQGQRRGPCWCVDRMGKSLPGSPDGNGSSSCPTGSSG

分子量

Approximately 36 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

IGFBP-6 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IGFBP-6 Protein, Human (HEK293, His)
目录号:
HY-P73141
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