IL-17A 蛋白, Human (T26A, sf9, His)

IL-17A is a homodimeric cytokine and plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides, cytokines, chemokines, and matrix metalloproteinases^[1][2]. IL-17A Protein, Human (sf9, His) is a recombinant human IL-17A protein with His tag at the N-terminus and is expressed in sf9 insect cells. It consists of 132 amino acids (G24-A155).

白细胞介素-17A (IL-17A)，也称为 CTLA-8，属于 IL-17 细胞因子家族。IL-17A 在记忆性 Th17 细胞中表达，是记忆性 CD4⁺ T 细胞的产物。IL-17A 也由多种免疫细胞产生，包括 CD8⁺ T 细胞、γδT 细胞、自然杀伤 T (NKT) 细胞、单核细胞和中性粒细胞^[1][2][3]。
人IL-17A与小鼠氨基酸序列同一性为63.23%，与大鼠氨基酸序列同一性为61.90%。
IL-17A 在宿主针对许多细菌和真菌病原体的防御机制以及过敏和自身免疫反应中发挥着关键作用。IL-17A 诱导抗菌肽（防御素和 S100 蛋白）、细胞因子（IL-6、G-CSF 和 GM-CSF）、趋化因子（CXCL1、CXCL5、IL-8、CCL2 和 CCL7）和基质的产生金属蛋白酶（MMP1、MMP3 和 MMP13）。IL-17A 通过促进中性粒细胞炎症而对病毒感染有害。IL-17A 是同型二聚体细胞因子，与 IL-17F 具有相似的生物活性。IL-17A与IL-17RA以高亲和力结合，IL-17RA是IL-17A生物活性所必需的。在肿瘤发生过程中，IL-17A 会募集骨髓源性抑制细胞 (MDSC) 来抑制抗肿瘤免疫。IL-17A 还可通过诱导 IL-6^[1][2] 促进体内肿瘤生长。
IL-17A 可用于研究自身免疫性疾病、感染和癌症^[1][4]。

IL-17A (1 ng/mL; 24 h) inhibits the expression of thymic stromal lymphopoietin (TSLP) in human primary keratinocytes^[5].
IL-17A (.1–1 ng/mL; -24 h) significantly potentiates TNF-α-induced IL-8 protein secretion and gene expression in a concentration- and time-dependent manner^[6].

Human

Sf9 insect cells

N-6*His

Q16552 (G24-A155, T26A)

3605  [NCBI]

15.9 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Chen K, et al. Interluekin-17A (IL17A). Gene. 2017 May 30;614:8-14.  [Content Brief]

  [2]. Iwakura Y, et al. The roles of IL-17A in inflammatory immune responses and host defense against pathogens. Immunol Rev. 2008 Dec;226:57-79.  [Content Brief]

  [3]. Cua DJ, et al. Innate IL-17-producing cells: the sentinels of the immune system. Nat Rev Immunol. 2010 Jul;10(7):479-89.  [Content Brief]

  [4]. Wright JF, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008 Aug 15;181(4):2799-805.  [Content Brief]

  [5]. Kinoshita H, et al. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol. 2009 Jan;123(1):179-86.  [Content Brief]

  [6]. Henness S, et al. IL-17A acts via p38 MAPK to increase stability of TNF-alpha-induced IL-8 mRNA in human ASM. Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1283-90.  [Content Brief]