IL-24 蛋白, Human (HEK293, His)

Interleukin-24 (IL-24) belongs to the IL10 family of cytokines that selectively induces apoptosis in cancer cells and is recognized during melanoma cell differentiation. IL-24 overexpression increases the expression of GADD family genes and induces cell apoptosis. IL-24 Protein, Human (HEK293, His) is the recombinant human-derived IL-24 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of IL-24 Protein, Human (HEK293, His) is 155 a.a., with molecular weight of ~35 kDa.

白细胞介素24（IL-24）属于细胞因子IL10家族，被鉴定为黑色素瘤细胞终末分化过程中诱导的基因。该基因编码的蛋白质表现出选择性诱导多种癌细胞凋亡的显着能力。IL-24 的过度表达会导致多个 GADD 家族基因的表达升高，这与诱导细胞凋亡有关。在黑色素瘤细胞中，IL-24 诱导丝裂原激活蛋白激酶 14 (MAPK7/P38) 和热休克 27kDa 蛋白 1 (HSPB2/HSP27) 磷酸化，但在正常永生黑色素细胞中未观察到这种效应。已报道了编码不同亚型的多种选择性剪接转录物变体，强调了 IL-24 复杂的调控环境。IL-24 在淋巴结 (RPKM 12.3)、脾 (RPKM 9.6) 和各种其他组织中偏向表达，在协调细胞凋亡和免疫反应中发挥着至关重要的作用。

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL­20 Rα and human IL­20 Rβ and the EC₅₀ is typically 0.05-0.25 ng/mL.

Human

HEK293

C-10*His

NP_001172085.1 (Q53-L207）

11009  [NCBI]

QEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL

Approximately 35 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.