1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. Multi-CSF/IL-3
  5. IL-3 蛋白, Mouse (HEK293)

IL-3 蛋白, Mouse (HEK293)

目录号: HY-P73207
COA 产品使用指南

IL-3蛋白由T淋巴细胞、肥大细胞和成骨细胞分泌。它在造血祖细胞中发挥重要的调节作用,并刺激成熟的嗜碱性粒细胞、嗜酸性粒细胞和单核细胞。除了造血作用外,它还通过抑制破骨细胞分化来支持神经细胞增殖、存活和骨稳态。IL-3 蛋白, Mouse (HEK293) 是重组的 IL-3 蛋白,由 HEK293 表达,不带标签。IL-3 蛋白, Mouse (HEK293) 全长 166 个氨基酸,分子量约为 ~15.7 kDa。

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规格 价格 是否有货 数量
10 μg ¥1850 In-stock
50 μg ¥5280 In-stock
100 μg   询价  

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描述

IL-3 protein is secreted by T lymphocytes, mast cells and osteoblasts. It plays an important regulatory role in hematopoietic progenitor cells and stimulates mature basophils, eosinophils and monocytes. In addition to hematopoiesis, it supports neuronal cell proliferation, survival, and bone homeostasis by inhibiting osteoclast differentiation. IL-3 Protein, Mouse (HEK293) is the recombinant mouse-derived IL-3 protein, expressed by HEK293 , with tag free. The total length of IL-3 Protein, Mouse (HEK293) is 166 a.a., with molecular weight of ~15.7 kDa.

研究背景

细胞因子 IL-3 主要由活化的 T 淋巴细胞、肥大细胞和成骨细胞分泌,在控制造血祖细胞向谱系限制细胞的产生和分化中发挥着至关重要的作用。此外,IL-3 可刺激成熟的嗜碱性粒细胞、嗜酸性粒细胞和单核细胞,促进其功能激活。除造血功能外,IL-3 还有助于神经细胞增殖和存活,并通过防止 NF-kappa-B 核易位和激活来抑制破骨细胞分化,从而参与骨稳态。从机制上讲,IL-3 通过由 IL3RA 亚基和信号转导亚基 IL3RB 组成的受体发挥其生物效应,导致 JAK2 激酶活性快速激活以及随后 STAT5 介导的转录编程。此外,IL-3 作为单体,通过激活 PI3K/AKT 和 ERK 介导的途径,有助于非造血系统中氧化应激下的细胞存活。

生物活性

Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells and the ED50 is 0.1-0.5 ng/mL.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P01586 (M1-C166)

基因 ID
蛋白结构
N-term
IL-3 (M1-C166)
Accession # P01586
C-term
同用名
Interleukin-3; IL-3; MCGF
分子量

Approximately 15.7 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-3 Protein, Mouse (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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