1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins
  3. Interleukin & Receptors T Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Epithelial cell CD Proteins Cytokine Receptors
  4. IL-6R IL-6R/CD126
  5. IL-6R alpha
  6. IL-6R alpha 蛋白, Human (Sf9)

IL-6R alpha 蛋白, Human (Sf9)

目录号: HY-P7377
COA 产品使用指南

IL-6R alphaIL-6R 的 α 亚基,也被其他白细胞介素受体共享。IL-6R alpha 可作为 IL-6 激动剂,参与包括 JAK/STAT、MAPK 和 Akt 在内的信号通路。IL-6R alpha 由 468 个氨基酸 (M1-R468) 组成,存在两个 N 端纤维连接蛋白 III 型结构域 (113-217 a.a, 218-316 a.a) 和可溶性形式 (1-365 a.a)。可溶性 IL-6R (sIL-6R) 可结合 IL-6 和二聚体化的 gp130 实现反式信号传导,并具有在特定免疫组织表达的特异性。IL-6R alpha 蛋白, Human (L20-D358) 由 Sf9 昆虫细胞表达,序列全长为 339 个氨基酸。

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描述

IL-6R alpha is a subunit alpha of IL-6 receptors, also shared by other interleukin receptors. IL-6R alpha acts as IL-6 agonist and involves in JAK/STAT, MAPK, and Akt signaling pathway[1][2]. IL-6R alpha/CD126, Human consists of 468 amino acids (M1-R468) with two fibronectin type-III-like domains contained in the N-terminal part (113-217 a.a, 218-316 a.a), and a soluble form (1-365 a.a). Soluble IL-6R (sIL-6R) binds IL-6 and dimerized gp130 to achieve trans signaling, and exhibits a tissue expression property in some immune systems. IL-6R alpha Protein, Human (L20-D358) is soluble form and expressed by Sf9 insect cells with a full length of the sequence of 339 amino acids.

研究背景

IL-6 兼具炎症因子和抗炎因子的作用,通过激活一系列下游信号级联反应,包括 gp130(二聚体)、JAK/STAT、MAPK 和 Akt[1]< super>[2]。
IL-6R α (IL-6Rα) 作为白细胞介素 6 受体的一部分,是一种 I 型跨膜糖蛋白,与 I 型跨膜信号形成复合物转导糖蛋白 130 (CD130) 并以低亲和力调节 IL-6 的生物活性[3]
人类 IL-6R α 蛋白的氨基酸序列差异很大来自小鼠 (54.07%) 和大鼠 (54.68%)。
IL-6R α 有 2 个亚型,包括 mIL6R(较长的一个)或 sIL6R(较短的一个):
mIL6R 是膜结合白细胞介素-6 受体,有潜力通过树突状细胞[4]的“簇信号传导”将幼稚 CD4+ T 细胞驱动至 Th17 谱系。
sIL-6R 是可溶性白细胞介素 6 受体亚基,通过蛋白水解作用从活化的 CD4+ T 细胞中的 IL-6R α (IL-6Rα) 上裂解下来,并作为 IL-6 激动剂。sIL-6R 结合膜结合的 IL6R 和亚基 IL6ST,通过 IL-6 反式信号传导激活再生和抗炎信号,并促进 IL-6 的促炎特性。IL-6R α 的水解也称为胞外域脱落[1]
IL-6R α 参与调节细胞生长和分化,在免疫反应调节中发挥重要作用。急性期反应和造血[5]
然而,IL-6R α 在肝脏和免疫系统的一些细胞中表现出组织表达特异性,从而导致 IL6 信号传导受到限制[6]
值得注意的是,IL-6R α 失调与多种疾病的发病机制有关,例如多发性骨髓瘤、自身免疫性疾病和前列腺癌[7][8]

体外研究
(In Vitro)

IL-6R alpha regulates IL-6 (10-6-103 ng/mL; 60 min at least) and triggers chemokinesis of tissue mast cells of rats[1].
sIL-6R (100 ng/mL; 48 h) shows no effect on adhesion molecules VCAM-1 and ICAM-1, but potently inhibits TNF-α induced VCAM-1 expression but not ICAM-1, by being combined with IL-6 (5 ng/mL) in U373-MG human astroglioma cells[2].
sIL-6R (100 ng/mL; 30 min) induce tyrosine phosphorylation of STAT-3 in U373-MG astroglioma cells and human astrocytes[2].
IL-6/sIL-6R complex (100 ng/mL; 24 h) induces NF-κB and STAT3 activation and Bcl-2 expression for human fibroblast-like synoviocytes in rheumatoid arthriti[3].

体内研究
(In Vivo)

IL-6R alpha exerts negative regulation on MAO-A activity to release angiogenic and invasive features of breast cancer cells from MAO-A inhibition[4].
sIL-6R (human), combinded with IL-6 (human), coexpressing in double-transgenic mice, inhibits mice growth and causes an extreme expansion of extramedullary hematopoietic progenitor cells compared with human IL-6 and human sIL-6R single-transgenic mice[5].
sIL-6R acts as a serum-binding protein for IL-6 and prolongs the plasma half life of IL-6[6].

生物活性

The ED50 is <50 ng/mL as measured by M1 cells, corresponding to a specific activity of >2 × 104 units/mg.

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

P08887 (L20-D358)

基因 ID
蛋白结构
N-term
IL-6Rα (L20-D358)
Accession # P08887
C-term
中文名
人 IL-6Rα 蛋白(Sf9)
同用名
rHuIL-6 Receptor α; gp80; CD126
氨基酸序列

HHHHHHDDDDKLAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQD

分子量

Approximately 50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-6R alpha Protein, Human (Sf9)
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