IL-9 蛋白, Mouse (sf9, His)

IL-9 is secreted by T helper 2 lymphocytes, mast cells, and NKT cells and plays an important role in antiparasitic immune responses, affecting intestinal permeability, adaptive immunity, and T cell subset differentiation. It promotes TH17 cell and mast cell proliferation through IL9R and IL2RG receptor activation, triggering JAK1 and JAK3 kinases and subsequent STAT-mediated transcription. IL-9 Protein, Mouse (sf9, His) is the recombinant mouse-derived IL-9 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of IL-9 Protein, Mouse (sf9, His) is 144 a.a., with molecular weight of ~15.6 kDa.

IL-9 是一种多功能细胞因子，主要由 T 辅助细胞 2 淋巴细胞以及肥大细胞或 NKT 细胞分泌，在针对寄生虫的免疫反应中发挥着至关重要的作用。除了抗寄生虫功能外，IL-9 还影响肠上皮通透性和适应性免疫。它在诱导特定 T 细胞亚群（例如产生 IL-17 的辅助 T 细胞（TH17））的分化中发挥着关键作用，并促进肥大细胞的增殖和分化。从机制上讲，IL-9 通过由 IL9R 亚基和信号转导亚基 IL2RG 组成的受体发挥其多种生物学作用。刺激该受体会导致 JAK1 和 JAK3 激酶活性快速激活，启动 STAT1、STAT3 和 STAT5 介导的转录程序。分化基因的诱导似乎仅由 STAT1 介导，而细胞免于凋亡的保护则取决于 STAT3 和 STAT5 的协同作用。IL-9 直接与 IL9R 和 IL2RG 相互作用，协调复杂的相互作用网络来调节免疫反应和细胞过程。

Measured in a cell proliferation assay using MC/9 mouse mast cells and the ED₅₀ is typically 0.2-2 ng/mL.

Mouse

Sf9 insect cells

C-His

P15247 (M1-P144)

16198  [NCBI]

MLVTYILASVLLFSSVLGQRCSTTWGIRDTNYLIENLKDDPPSKCSCSGNVTSCLCLSVPTDDCTTPCYREGLLQLTNATQKSRLLPVFHRVKRIVEVLKNITCPSFSCEKPCNQTMAGNTLSFLKSLLGTFQKTEMQRQKSRP

Approximately 15.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.