1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Integrin
  5. Integrin alpha 6 beta 1
  6. Integrin alpha 6 beta 1 蛋白, Human (HEK293, Flag-His)

Integrin alpha 6 beta 1 蛋白, Human (HEK293, Flag-His)

目录号: HY-P73871
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Human Laminin alpha 4 is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human Integrin alpha 6 beta 1. The ED50 for this effect is 7.473 nM.

种属

Human

表达系统

HEK293

标签

C-His;C-Flag

蛋白编号

P23229-3 (F24-S1011)&P05556 (Q21-D728)

基因 ID

3655  [NCBI]&3688  [NCBI]

蛋白结构
N-term
ITGA6 (F24-S1011)
Accession # P23229-3
Flag
C-term
N-term
ITGB4 (Q21-D728)
Accession # P05556-1
His
C-term
同用名
CD49f; Integrin alpha 6 beta 1; ITGA6B; VLA-6
分子量

Approximately 110&130 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His)
目录号:
HY-P73871
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