1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. JNK3 蛋白, Human (His)

JNK3 蛋白, Human (His)

目录号: HY-P701791
产品使用指南

JNK3 是神经元过程中关键的丝氨酸/苏氨酸蛋白激酶,调节增殖、分化、迁移和凋亡。通过 MAP2K4/MKK4 和 MAP2K7/MKK7 激活会导致 JNK3 磷酸化,进而磷酸化 AP-1 成分(如 JUN 和 ATF2),从而调节 AP-1 转录活性。JNK3 蛋白, Human (His) 是重组的 JNK3 蛋白,由 E. coli 表达,带有 N-6*His 标签。JNK3 蛋白, Human (His) 全长 423 个氨基酸。

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描述

JNK3 is a critical serine/threonine protein kinase in neuronal processes, regulating proliferation, differentiation, migration, and apoptosis. Activation through MAP2K4/MKK4 and MAP2K7/MKK7 leads to phosphorylation of JNK3, which in turn phosphorylates AP-1 components such as JUN and ATF2, thereby regulating AP-1 transcriptional activity. JNK3 Protein, Human (His) is the recombinant human-derived JNK3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of JNK3 Protein, Human (His) is 423 a.a., .

研究背景

JNK3 是一种丝氨酸/苏氨酸蛋白激酶,在多种神经元过程中发挥着关键作用,包括增殖、分化、迁移和程序性细胞死亡。细胞外刺激(例如促炎细胞因子或身体应激)激活应激激活蛋白激酶/c-Jun N 末端激酶 (SAP/JNK) 信号通路,涉及双特异性激酶 MAP2K4/ 磷酸化和激活 JNK3 MKK4 和 MAP2K7/MKK7。在这个级联中,JNK3 磷酸化关键转录因子,特别是 AP-1 的组件,如 JUN 和 ATF2,从而调节 AP-1 转录活性。JNK3 通过调节信号通路在神经元凋亡中发挥关键作用,并且它磷酸化神经元微管调节因子 STMN2。此外,在神经元分化过程中淀粉样蛋白-β 前体蛋白/APP 信号传导的调节中,JNK3 磷酸化 APP。此外,JNK3 有助于螺旋神经节神经元中的神经突生长,并使 CLOCK-BMAL1 异二聚体磷酸化,从而影响生物钟的光调节。值得注意的是,JNK3 磷酸化 JUND,并且这种磷酸化在 MEN1 存在的情况下受到抑制。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P53779 (M1-S423)

基因 ID

5602

蛋白结构
N-term
6*His
JNK3 (M1-S423)
Accession # P53779
C-term
同用名
MAPK10; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

JNK3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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JNK3 Protein, Human (His)
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HY-P701791
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