1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. Fibroblast Growth Factor 7 (FGF-7)
  6. KGF/FGF-7 蛋白, Human (HEK293, His)

KGF/FGF-7 蛋白, Human (HEK293, His)

目录号: HY-P70673
COA 产品使用指南

KGF/FGF-7 蛋白协调胚胎发育并调节细胞增殖和分化等基本过程。它的关键作用延伸到正常的分支形态发生。KGF/FGF-7 蛋白, Human (HEK293, His) 是重组的 KGF/FGF-7 蛋白,由 HEK293 表达,带有 C-6*His 标签。KGF/FGF-7 蛋白, Human (HEK293, His) 全长 163 个氨基酸,分子量为 20-28 kDa。

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3.  试用装只面向终端客户

规格 价格 是否有货 数量
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2 μg ¥1040 In-stock
10 μg ¥2900 In-stock
50 μg ¥8700 In-stock
100 μg ¥14790 In-stock
> 100 μg   询价  

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描述

KGF/FGF-7 proteins coordinate embryonic development and regulate basic processes such as cell proliferation and differentiation. Its critical role extends to normal branching morphogenesis. KGF/FGF-7 Protein, Human (HEK293, His) is the recombinant human-derived KGF/FGF-7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of KGF/FGF-7 Protein, Human (HEK293, His) is 163 a.a., with molecular weight of 20-28 kDa.

研究背景

KGF/FGF-7 蛋白在协调胚胎发育中发挥着至关重要的作用,对细胞增殖和分化等基本过程具有调节控制作用。它的重要贡献延伸到正常分支形态发生的复杂领域,其中 KGF/FGF-7 发挥着关键作用。作为一种对角质形成细胞具有特异性活性的生长因子,它是控制正常上皮细胞增殖的潜在主要旁分泌效应物。该蛋白建立了关键的相互作用,与 FGFBP1 和 FGFR2 形成复合物,并且其与成纤维细胞生长因子 (FGF) 及其受体的结合亲和力通过硫酸乙酰肝素糖胺聚糖增强,在这些分子相互作用中充当关键的辅助受体。

生物活性

1. The cell proliferation assay using HaCaT cells has an ED50 value of 1.35-10.94 ng/mL.
2. Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 this effect is <47 ng/mL, corresponding to a specific activity is >2.13×104 units/mg.

  • Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 this effect is 33.63 ng/mL, corresponding to a specific activity is 2.9735×104 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P21781 (C32-T194)

基因 ID
蛋白结构
N-term
FGF-7 (C32-T194)
Accession # P21781
6*His
C-term
同用名
Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7
氨基酸序列

CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT

分子量

20-28 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
KGF/FGF-7 Protein, Human (HEK293, His)
目录号:
HY-P70673
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