Leukocidin-F subunit/LukF 蛋白, S. aureus (Myc, His-SUMO)

Leukocidin-F subunit/LukF protein induces cytotoxic changes in polymorphonuclear leukocytes, while gamma-hemolysin, with components H-gamma-I and H-gamma-II identical to F, causes hemolysis in red blood cells. These proteins collectively contribute to pathogenic mechanisms by exerting cytotoxic effects on immune cells and inducing hemolysis in red blood cells. Leukocidin-F subunit/LukF Protein, S. aureus (Myc, His-SUMO) is the recombinant Staphylococcus aureus-derived Leukocidin-F subunit/LukF protein, expressed by E. coli , with N-His, C-Myc, N-SUMO labeled tag. The total length of Leukocidin-F subunit/LukF Protein, S. aureus (Myc, His-SUMO) is 298 a.a., with molecular weight of ~54.0 kDa.

Leukocidin-F 亚基/LukF 蛋白可诱导多形核白细胞发生细胞毒性变化，而 γ-溶血素则可导致红细胞溶血。杀白细胞素由两种蛋白质成分组成：F 和 S。此外，γ-溶血素由两种蛋白质成分组成，即与 F 相同的 H-gamma-I 和 H-gamma-II。这些蛋白质通过对免疫细胞发挥细胞毒性作用并引起红细胞溶血，从而促进整体致病机制。

Staphylococcus aureus

E. coli

N-His;C-Myc;N-SUMO

P31715 (26A-323K)

/

AEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNAVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTLSRNTNYKNVGWGVEAHKIMNGWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDRAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK

Approximately 54.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.