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Lipoate-protein ligase A/LplA 蛋白, E.coli (His-SUMO)

目录号: HY-P71524
COA 产品使用指南

硫辛酸蛋白连接酶 A (LplA) 是参与硫辛酸(重要辅助因子)激活和转移至硫辛酸依赖性酶的硫辛酸结构域的关键酶。LplA 催化外源硫辛酸的 ATP 依赖性激活,将其转化为硫辛酸-AMP 作为中间体。Lipoate-protein ligase A/LplA 蛋白, E.coli (His-SUMO) 是重组的 脂酸蛋白连接酶 A/LplA 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。Lipoate-protein ligase A/LplA 蛋白, E.coli (His-SUMO) 全长 338 个氨基酸,分子量约为 ~53.9 kDa。

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描述

Lipoic acid protein ligase A (LplA) is a key enzyme involved in the activation and transfer of lipoic acid, an important cofactor, to the lipoyl domain of lipoic acid-dependent enzymes. LplA catalyzes the ATP-dependent activation of exogenous lipoic acid, converting it to lipoyl-AMP as an intermediate. Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Lipoate-protein ligase A/LplA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is 338 a.a., with molecular weight of ~53.9 kDa.

研究背景

硫辛酸蛋白连接酶 A (LplA) 是一种关键酶,参与硫辛酸(一种重要的辅助因子)的激活和转移到硫辛酸依赖性酶的硫辛酰结构域上。LplA 催化外源硫辛酸的 ATP 依赖性激活,将其转化为硫辛酰-AMP,作为中间体。然后,LplA 利用这种活化的硫辛酰-AMP 将硫辛酰部分转移到靶蛋白上。通过促进这一转移过程,LplA 确保硫辛酸依赖性酶的正常功能,这些酶参与各种重要的代谢途径,例如柠檬酸循环和脂肪酸代谢。了解 LplA 介导的硫辛酸活化和转移的机制和调节可以为细胞能量代谢和代谢稳态的维持提供见解。

种属

E.coli

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

C4ZT68 (M1-R338)

基因 ID

/

蛋白结构
N-term
6*His-SUMO
LplA (M1-R338)
Accession # C4ZT68
C-term
同用名
lplA; BWG_4078Lipoate-protein ligase A; EC 6.3.1.20; Lipoate--protein ligase
氨基酸序列

MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR

分子量

Approximately 53.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO)
目录号:
HY-P71524
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