MAG/Siglec-4a 蛋白, Human (HEK293, His)

MAG/Siglec-4a is an adhesion molecule that binds to neuronal gangliosides, RTN4R, and RTN4RL2, promoting myelination interactions. Although not important for initial myelination, it may maintain axonal myelination and protect motor neurons, especially after injury, through interactions with RTN4R and RTN4RL2. MAG/Siglec-4a Protein, Human (HEK293, His) is the recombinant human-derived MAG/Siglec-4a protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAG/Siglec-4a Protein, Human (HEK293, His) is 497 a.a., with molecular weight of 84-108 kDa.

MAG/Siglec-4a 蛋白作为粘附分子，通过与神经元唾液酸神经节苷脂以及糖蛋白 RTN4R 和 RTN4RL2 结合，促进髓鞘形成细胞和神经元之间的相互作用。虽然 MAG/Siglec-4a 对于初始髓鞘形成不是必需的，但它似乎在维持正常轴突髓鞘形成和保护运动神经元免于凋亡方面发挥着至关重要的作用，特别是在损伤后。这种保护作用可能是通过与神经元 RTN4R 和 RTN4RL2 的相互作用介导的。在成人中，MAG/Siglec-4a 需要防止有髓轴突变性，可能依赖于与轴突细胞膜上的神经节苷脂的结合。作为神经突生长的负调节因子，MAG/Siglec-4a 通过优先与 α-2,3 连接唾液酸结合并与 RTN4R、RTN4RL2 和神经节苷脂相互作用来抑制轴突纵向生长和生长。该蛋白以单体和同型二聚体的形式存在，其相互作用延伸至 BSG 的亚型 2，从而使其在调节神经元反应和轴突动力学方面发挥着复杂的作用。

Human

HEK293

C-6*His

P20916 (G20-P516)

4099  [NCBI]

GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

84-108 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.