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  3. OSTM1 蛋白, Mouse (HEK293, His)

OSTM1 蛋白, Mouse (HEK293, His)

目录号: HY-P77117
COA 产品使用指南

OSTM1 蛋白对于破骨细胞和黑素细胞的成熟和功能至关重要,与 α 亚基 (CLCN7) 形成异聚体以创建氯离子通道 7。OSTM1 蛋白, Mouse (HEK293, His) 是重组的 OSTM1 蛋白,由 HEK293 表达,带有 C-His 标签。OSTM1 蛋白, Mouse (HEK293, His) 全长 254 个氨基酸,分子量约为 ~29.7 KDa。

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5 μg ¥550 In-stock
10 μg ¥950 In-stock
50 μg ¥2650 In-stock
100 μg ¥4500 In-stock
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描述

OSTM1 Protein is crucial for osteoclast and melanocyte maturation and function, forming heteromers with alpha subunits (CLCN7) to create chloride channel 7. OSTM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived OSTM1 protein, expressed by HEK293 , with C-His labeled tag. The total length of OSTM1 Protein, Mouse (HEK293, His) is 254 a.a., with molecular weight of ~29.7 KDa.

研究背景

OSTM1 蛋白对于破骨细胞和黑素细胞的成熟和正常功能至关重要。它与 α 亚基 (CLCN7) 形成异聚体以创建氯离子通道 7。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q8BGT0 (A35-S288)

基因 ID

14628  [NCBI]

蛋白结构
N-term
OSTM1 (A35-S288)
Accession # Q8BGT0
His
C-term
同用名
Osteopetrosis-associated transmembrane protein 1; Chloride channel 7 beta subunit; GL
氨基酸序列

ALPFTSSRHPGFADLLSEQQLLEVQDLTLSLLQGGGLGPLSLLPPDLPDLEPECRELLMDFANSSAELTACMVRSARPVRLCQTCYPLFQQVAIKMDNISRNIGNTSEGPRCGGSLLTADRMQIVLMVSEFFNSTWQEANCANCLTNNGEDLSNNTEDFLSLFNKTLACFEHNLQGHTYSLLPPKNYSEVCRNCKEAYKNLSLLYSQMQKLNGLENKAEPETHLCIDVEDAMNITRKLWSRTFNCSVTCSDTVS

分子量

Approximately 30-60 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

OSTM1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OSTM1 Protein, Mouse (HEK293, His)
目录号:
HY-P77117
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