1. 重组蛋白
  2. CD Antigens Receptor Proteins Biotinylated Proteins
  3. Platelet CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. P-Selectin/CD62P Selectin
  5. P-Selectin
  6. P-selectin 蛋白, Mouse (Biotinylated, HEK293, Avi-His)

P-selectin 蛋白, Mouse (Biotinylated, HEK293, Avi-His)

目录号: HY-P72408
COA 产品使用指南

P-选择素是骨髓细胞上的 Ca(2+) 依赖性受体,与中性粒细胞和单核细胞上的碳水化合物特异性结合。它通过识别唾液酸-Lewis X 介导活化的内皮细胞或血小板与白细胞之间的相互作用。P-selectin 蛋白, Mouse (Biotinylated, HEK293, Avi-His) 是重组的 P-选择素蛋白,由 HEK293 表达,带有 C-Avi, C-6*His 标签。P-selectin 蛋白, Mouse (Biotinylated, HEK293, Avi-His) 全长 668 个氨基酸,分子量为 102-122 kDa。

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重组蛋白定制服务

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥3000 In-stock
100 μg ¥7500 In-stock
> 100 μg   询价  

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描述

P-selectin is a Ca(2+)-dependent receptor on myeloid cells that specifically binds to carbohydrates on neutrophils and monocytes. It mediates interactions between activated endothelial cells or platelets and leukocytes by recognizing sialic acid-Lewis X. P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His) is the recombinant mouse-derived P-selectin protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His) is 668 a.a., with molecular weight of 102-122 kDa.

研究背景

P-选择素蛋白是一种在骨髓细胞上发现的 Ca(2+) 依赖性受体,它与中性粒细胞和单核细胞上的碳水化合物特异性结合。它在介导活化的内皮细胞或血小板与白细胞之间的相互作用中起着至关重要的作用。P-选择素识别的配体是唾液酸-Lewis X。P-选择素通过与 SELPLG 相互作用,促进炎症初始阶段白细胞在血管表面快速滚动。它还与 SNX17 相互作用。此外,P-选择素与 SELPLG/PSGL1 和 PODXL2 相互作用,促进中性粒细胞粘附和白细胞滚动。这种相互作用需要 SELPLG 和 PODXL2 上存在唾液酸-Lewis X 表位,以及 SELPLG 上特定的酪氨酸硫酸化。

种属

Mouse

表达系统

HEK293

标签

C-Avi;C-6*His

蛋白编号

Q01102 (W42-A709)

基因 ID

20344  [NCBI]

蛋白结构
N-term
P-selectin (W42-A709)
Accession # Q01102
Avi-6*His
C-term
同用名
CD62 antigen-like family member P; GMP-140; LECAM3; PADGEM
氨基酸序列

WTYNYSTKAYSWNNSRVFCRRHFTDLVAIQNKNEIAHLNDVIPFFNSYYWIGIRKINNKWTWVGTNKTLTEEAENWADNEPNNKKNNQDCVEIYIKSNSAPGKWNDEPCFKRKRALCYTASCQDMSCSNQGECIETIGSYTCSCYPGFYGPECEYVKECGKVNIPQHVLMNCSHPLGEFSFNSQCTFSCAEGYELDGPGELQCLASGIWTNNPPKCDAVQCQSLEAPPHGTMACMHPIAAFAYDSSCKFECQPGYRARGSNTLHCTGSGQWSEPLPTCEAIACEPPEIPIHGSMDCVPSTGTFGYNSSCTFLCAEGFVLKGNDAIQCADSGQWTAPAPFCEALQCPEFPVPSKAQVNCSDPFGTLTYQSVCSFSCDEGSLLVGASVIRCLATGHWNGAPPECQAVSCAPMLSPENGSMTCVQPLGNSTYKSTCQFMCDEGFYLSGPERLDCSPSGHWTGTPPTCEAIKCPGIFAPEQGNLDCSHVHGEFGVGSICHFSCNEDFELLGSENVECTVSGRWSAPPPTCKGITSLPAPAVRCPALTTPGQGTMSCQHHLGSFGPNTTCYFGCKTGFTLRGANSLRCRASGQWTAVTPMCRAVKCSELHMDTAVAMNCSNPWGNFSYGSTCTFQCPEGQSLNGSVRATCREDGHWSDAMPTCQAGTLTIQEA

分子量

102-122 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
P-selectin Protein, Mouse (Biotinylated, HEK293, Avi-His)
目录号:
HY-P72408
需求量: