P-Selectin 蛋白, Mouse (HEK293, Fc)

P-selectin is a Ca(2+)-dependent receptor on myeloid cells that specifically binds to carbohydrates on neutrophils and monocytes. It mediates interactions between activated endothelial cells or platelets and leukocytes by recognizing sialic acid-Lewis X. P-Selectin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived P-Selectin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of P-Selectin Protein, Mouse (HEK293, Fc) is 668 a.a., with molecular weight of ~135 kDa.

P-选择素蛋白是一种在骨髓细胞上发现的 Ca(2+) 依赖性受体，它与中性粒细胞和单核细胞上的碳水化合物特异性结合。它在介导活化的内皮细胞或血小板与白细胞之间的相互作用中起着至关重要的作用。P-选择素识别的配体是唾液酸-Lewis X。P-选择素通过与 SELPLG 相互作用，促进炎症初始阶段白细胞在血管表面快速滚动。它还与 SNX17 相互作用。此外，P-选择素与 SELPLG/PSGL1 和 PODXL2 相互作用，促进中性粒细胞粘附和白细胞滚动。这种相互作用需要 SELPLG 和 PODXL2 上存在唾液酸-Lewis X 表位，以及 SELPLG 上特定的酪氨酸硫酸化。

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED₅₀ this effect is 5.013 μg/mL, corresponding to a specific activity is 199.48 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.The ED₅₀ this effect is 5.013 μg/mL, corresponding to a specific activity is 199.48 units/mg.

Mouse

HEK293

C-hFc

Q01102 (W42-A709)

20344  [NCBI]

WTYNYSTKAYSWNNSRVFCRRHFTDLVAIQNKNEIAHLNDVIPFFNSYYWIGIRKINNKWTWVGTNKTLTEEAENWADNEPNNKKNNQDCVEIYIKSNSAPGKWNDEPCFKRKRALCYTASCQDMSCSNQGECIETIGSYTCSCYPGFYGPECEYVKECGKVNIPQHVLMNCSHPLGEFSFNSQCTFSCAEGYELDGPGELQCLASGIWTNNPPKCDAVQCQSLEAPPHGTMACMHPIAAFAYDSSCKFECQPGYRARGSNTLHCTGSGQWSEPLPTCEAIACEPPEIPIHGSMDCVPSTGTFGYNSSCTFLCAEGFVLKGNDAIQCADSGQWTAPAPFCEALQCPEFPVPSKAQVNCSDPFGTLTYQSVCSFSCDEGSLLVGASVIRCLATGHWNGAPPECQAVSCAPMLSPENGSMTCVQPLGNSTYKSTCQFMCDEGFYLSGPERLDCSPSGHWTGTPPTCEAIKCPGIFAPEQGNLDCSHVHGEFGVGSICHFSCNEDFELLGSENVECTVSGRWSAPPPTCKGITSLPAPAVRCPALTTPGQGTMSCQHHLGSFGPNTTCYFGCKTGFTLRGANSLRCRASGQWTAVTPMCRAVKCSELHMDTAVAMNCSNPWGNFSYGSTCTFQCPEGQSLNGSVRATCREDGHWSDAMPTCQAGTLTIQEA

Approximately 135 kDa

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  Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.